LOCUS       sequence-29900-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  TITLE     Howley Lab Plasmids
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p6268 pDEST-59-His-Trx-HPV16E6
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..414
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MASDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEF
                     LDANLA"
     CDS             421..438
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    442..466
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtngaattcc ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag ccatatggct agcgataaaa ttattcacct gactgacgac
      121 agttttgaca cggatgtact caaagcggac ggggcgatcc tcgtcgattt ctgggcagag
      181 tggtgcggtc cgtgcaaaat gatcgccccg attctggatg aaatcgctga cgaatatcag
      241 ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc ctggcactgc gccgaaatat
      301 ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa
      361 gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggcccgagct
      421 cttgttccac gtggttctgt cacaagtttg tacaaaaaag caggctccgc ggccgccccc
      481 ttcaccatgc accaaaagag aactgcaatg tttcaggacc cacaggagcg acccagaaag
      541 ttaccacagt tatgcacaga gctgcaaaca actatacatg atataatatt agaatgtgtg
      601 tactgcaagc aacagttact gcgacgtgag gtatatgact ttgcttttcg ggatttatgc
      661 atagtatata gagatgggaa tccatatgct gtatgtgata aatgtttaaa gttttattct
      721 aaaattagtg agtatagacn tattgttata gtttgtatgg acacntagac agcatacaca
      781 ccgtttggcn tt
//
