LOCUS       sequence-299004-       10261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acetylcholine sensor for lenti virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Borden PM, Zhang P, Shivange AV, Marvin JS, Cichon J, Dan C, 
            Podgorski K, Figueiredo A, Novak O, Tanimoto M, Shigetomi E, Lobas 
            MA, Kim H, Zhu PK, Zhang Y, Zheng WS, Fan C, Wang G, Xiang B, Gan L,
            Zhang G, Guo K, Lin L, Cai Y, Yee AG, Aggarwal A, Ford CP, Rees DC, 
            Dietrich D, Khakh BS, Dittman JS, Gan W, Koyama M, Jayaraman V, 
            Cheer JF, Lester HA, Zhu JJ, Looger LL
  TITLE     A fast genetically encoded fluorescent sensor for faithful in vivo 
            acetylcholine detection in mice, fish, worms and flies
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 10261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020"
COMMENT     Sequence Label: pLenti-hSynapsin-iAchSnFR
FEATURES             Location/Qualifiers
     source          1..10261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     sig_peptide     471..530
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     983..1004
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1107..1128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2115..2144
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2148..2294
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    2322..2910
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2375..2395)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2793..2804)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2913..2929)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2914..2930)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3435..3615
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3641..3658)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3647..3871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3917..4345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4004..4023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4214..4235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4354..4374)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4359..4688
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4539..4674
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4601..4620
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4736..4783
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4802..5176
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5306..5427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5343..5362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5397..5416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5476..5492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5476..5492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5489..5511)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5500..5516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5524..5530,5531..5548,5549..5554))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5569..5590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5707..5724)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5878..6466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5958..5977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6637..7428,7429..7497))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7260..7279
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7498..7602)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7677..7696)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7868..8247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8249..8447
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8402..8422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8465..8645
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8692..8817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9310..9543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9728..9772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10070..10187
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggtac cggatccacc atggagacag
      481 acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt gacagatctg
      541 cgaacgacac cgtagttgtg ggctcgatca tctttacaga agggattatc gtcgcaaaca
      601 tggtggcaga gatgattgag gcgcatacag accttaaggt ggttcgcaaa ctgaaccttg
      661 gcggggtcaa cgttaacttt gaagccatta aacgcggagg tgcgaataat ggtattgaca
      721 tttacgtgga gtacactggg cacggtcttg tggatattct ggggttcccg gagccgaacg
      781 tctatatcac cgccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
      841 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      901 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgtg ctgagcaaag
      961 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1021 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatgagc aagggcgagg
     1081 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1141 agttcagcgt gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt
     1201 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1261 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1321 ccgccatgcc cgaaggctac gtccaggagc gcaccatcag cttcaaggac gacggcacct
     1381 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1441 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactttc
     1501 cgccgcccgc gactactgat ccagaaggtg catacgaaac cgtgaagaag gagtacaaac
     1561 gtaaatggaa tattgtatgg ctcaaaccac tgggattcaa caatacgtat acgcttaccg
     1621 ttaaagacga actggcgaaa cagtataacc ttaaaacctt cagtgactta gcgaaaatct
     1681 cggataagct gattctgggt gcaacgatgt tctttttaga agggcccgat ggttacccag
     1741 gcctgcaaaa actgtacaat ttcaaattca agcacaccaa aagcatggac atgggtattc
     1801 gctataccgc cattgataat aacgaagttc aggtaattga tgcctgggcc actgatggct
     1861 tgctggtgag ccacaaatta aaaattctgg aggatgataa agcgttcttc ccgccgtatt
     1921 atgctgcccc catcatccgt caggatgtct tagataagca tcctgaactg aaggacgtgc
     1981 tgaacaaact cgcgaatcaa atttcactgg aagaaatgca gaaactgaat tacaaggtgg
     2041 acggtgaggg tcaggaccca gcgaaagtag ctaaggagtt tttgaaagag aaaggtttaa
     2101 ttctgcaggt cgacgaacaa aaactcatct cagaagagga tctgaatgct gtgggccagg
     2161 acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg gtgatctcag
     2221 ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc atgctttggc
     2281 agaagaagcc acgttaggaa ttcgatatca agcttatcga taatcaacct ctggattaca
     2341 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2401 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2461 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2521 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2581 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2641 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2701 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     2761 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2821 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2881 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     2941 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     3001 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     3061 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3121 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     3181 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     3241 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     3301 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     3361 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     3421 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3481 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3541 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3601 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3661 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3721 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3781 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3841 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3901 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3961 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4021 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4081 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4141 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4201 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4261 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4321 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4381 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4441 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4501 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4561 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4621 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4681 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     4741 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     4801 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     4861 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     4921 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     4981 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     5041 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     5101 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     5161 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     5221 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5281 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5341 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5401 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     5461 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5521 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5581 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5641 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6301 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6541 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6601 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6661 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6721 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6781 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6841 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6901 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6961 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7021 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7081 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7141 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7201 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7261 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7321 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7381 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7441 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7501 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7561 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7621 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     7681 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     7741 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     7801 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     7861 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     7921 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     7981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     8041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     8101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     8161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     8221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     8281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     8341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     8401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
     8461 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8521 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8581 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8641 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8701 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8761 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8821 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8881 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8941 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9001 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9061 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9121 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     9181 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9241 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9301 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9361 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9421 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9481 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9541 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9601 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9661 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9721 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9781 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9841 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9901 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     9961 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    10021 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
    10081 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    10141 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10201 gggacagcag agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat
    10261 g
//
