LOCUS       sequence-29901-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  TITLE     Howley Lab Plasmids
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p6272 pDEST-60-GST-HPV16E6
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    32..56
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(578..602)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             604..621
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggatgcagc cttgttccac gtggttctgt cacaagtttg tacaaaaaag caggctccgc
       61 ggccgccccc ttcaccatgc accaaaagag aactgcaatg tttcaggacc cacaggagcg
      121 acccagaaag ttaccacagt tatgcacaga gctgcaaaca actatacatg atataatatt
      181 agaatgtgtg tactgcaagc aacagttact gcgacgtgag gtatatgact ttgcttttcg
      241 ggatttatgc atagtatata gagatgggaa tccatatgct gtatgtgata aatgtttaaa
      301 gttttattct aaaattagtg agtatagaca ttattgttat agtttgtatg gaacaacatt
      361 agaacagcaa tacaacaaac cgttgtgtga tttgttaatt aggtgtatta actgtcaaaa
      421 gccactgtgt cctgaagaaa agcaaagaca tctggacaaa aagcaaagat tccataatat
      481 aaggggtcgg tggaccggtc gatgtatgtc ttgttgcaga tcatcaagaa cacgtagaga
      541 aacccagctg taagcggccg caagggtggg cgcgccgacc cagctttctt gtacaaagtg
      601 gttcatcacc accatcatca ctaagtgatt aacctaggct gctgccaccg ctga
//
