LOCUS       sequence-29902-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  TITLE     Howley Lab Plasmids
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p6272 pDEST-60-GST-HPV16E6
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..691
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     649..671
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             704..721
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggaaatattt tgtttacttt aagaaggaga tataccatgn tcccctatac taggttattg
       61 gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata
      121 tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt
      181 gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc
      241 tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga
      301 gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag
      361 aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga
      421 aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt
      481 aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat
      541 gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca
      601 aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc
      661 cacgtttggt ggtggcgacc atcctccaaa atcggatgca agccttgttc cacgtggttc
      721 tgtcacagtt tgtacaaaaa gcagctccgc gccgcccctt cacatgcaca anaac
//
