LOCUS       sequence-29903-e         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Auer S, Sturzebecher AS, Juttner R, Santos-Torres J, Hanack C, Frahm
            S, Liehl B, Ibanez-Tallon I
  TITLE     Silencing neurotransmission with membrane-tethered toxins.
  JOURNAL   Nat Methods. 2010 Mar;7(3):229-36. doi: 10.1038/nmeth.1425. Epub 
            2010 Feb 7.
  PUBMED    20139968
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1425"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "229-36"
COMMENT     Sequence Label: pFU-cMVIIA-PE
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(47..193)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(266..289)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(368..412)
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     protein_bind    427..460
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggccggtg gatcctacgt ggtttcttct
       61 gccaaagcat gatgaggatg ataagggaga tgatggtgag caccaccagg gccaggatgg
      121 ctgagatcac caccacctta aagggcaagg agtgtggcac cacgatgacc tcctgcgtgt
      181 cctggcccac agcaactcga gtaccatccc cgttgccgtt tccgttacca ttcccgttgc
      241 catttccgtt accagcggcc gcaagcttgt cgtcatcgtc tttgtaatcg atgcacttac
      301 cggacctaca cgatccggtg caacagtcat acatgaggcg ggagcacttc gcgcccttgc
      361 ctttgcaagc aactgcagct ccaacaagag ctaggatcag aagtgcagac atggtggcac
      421 cggtatataa cttcgtataa tgtatgctat acgaagttat gtcactcact cagtctagag
      481 tcgcgagctc tacaggaaca ggtggtggcg gccctcggtg cgctcgtact gctccacgat
      541 ggtgtagtcc ttgttgtggg aggtgatgtc cagcttggcg tccacgtagt agtagccg
//
