LOCUS       sequence-29904-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Auer S, Sturzebecher AS, Juttner R, Santos-Torres J, Hanack C, Frahm
            S, Liehl B, Ibanez-Tallon I
  TITLE     Silencing neurotransmission with membrane-tethered toxins.
  JOURNAL   Nat Methods. 2010 Mar;7(3):229-36. doi: 10.1038/nmeth.1425. Epub 
            2010 Feb 7.
  PUBMED    20139968
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1425"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "229-36"
COMMENT     Sequence Label: pFU-MVIIA-PC
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..245)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(318..341)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(420..464)
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     primer_bind     complement(655..674)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
       61 atgttatcct cctcgccctt gctcaccata cggatcctac gtggtttctt ctgccaaagc
      121 atgatgagga tgataaggga gatgatggtg agcaccacca gggccaggat ggctgagatc
      181 accaccacct taaagggcaa ggagtgtggc accacgatga cctcctgcgt gtcctggccc
      241 acagcaactc gagtaccatc cccgttgccg tttccgttac cattcccgtt gccatttccg
      301 ttaccagcgg ccgcaagctt gtcgtcatcg tctttgtaat cgatgcactt accggaccta
      361 cacgatccgg tgcaacagtc atacatgagg cgggagcact tcgcgccctt gcctttgcaa
      421 gcaactgcag ctccaacaag agctaggatc agaagtgcag acataccggt acccggggat
      481 cctctagagt cgacctgcag cccaagcttc gtctaacaaa aaagccaaaa acggccagaa
      541 tttagcggac aatttactag tctaacactg aaaattacat attgacccaa atgattacat
      601 ttcnnaaggt gcctaaaaaa cttcacaaaa cacactcgcc aaccccganc gcatagttca
      661 aaaccggagc ttcagctact taagaagata ggtacataaa accgaccaaa gaaactgacg
      721 cctcacttat ccctcccctc acca
//
