LOCUS       sequence-29905-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Auer S, Sturzebecher AS, Juttner R, Santos-Torres J, Hanack C, Frahm
            S, Liehl B, Ibanez-Tallon I
  TITLE     Silencing neurotransmission with membrane-tethered toxins.
  JOURNAL   Nat Methods. 2010 Mar;7(3):229-36. doi: 10.1038/nmeth.1425. Epub 
            2010 Feb 7.
  PUBMED    20139968
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1425"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "229-36"
COMMENT     Sequence Label: pFU-no toxin-PE
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(47..193)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(266..289)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(293..337)
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     primer_bind     complement(528..547)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 ctcctcggcc cttgctcacc atggtggcga ccggccggtg gatcctacgt ggtttcttct
       61 gccaaagcat gatgaggatg ataagggaga tgatggtgag caccaccagg gccaggatgg
      121 ctgagatcac caccacctta aagggcaagg agtgtggcac cacgatgacc tcctgcgtgt
      181 cctggcccac agcaactcga gtaccatccc cgttgccgtt tccgttacca ttcccgttgc
      241 catttccgtt accagcggcc gcaagcttgt cgtcatcgtc tttgtaatcg atagcaactg
      301 cagctccaac aagagctagg atcagaagtg cagacatacc ggtacccggg gatcctctag
      361 agtcgacctg cagcccaagc ttcgtctaac aaaaaagcca aaaacggcca gaatttagcg
      421 gacaatttac tagtctaaca ctgaaaatta catattgacc caaatgatta catttcaaaa
      481 ggtgcctaaa aaacttcaca aaacacactc gccaaccccg agcgcatagt tcaaaaccgg
      541 agcttcagct acttaagaag ataggtacat aaaaccgacc aaagaaactg acgcctcact
      601 tatccctccc ctcaccagag gtccggcgcc tgtcgattca ngagagccta ccctaggccc
      661 gaaccctgcg tcctgcgacg gagaaaagcc taccgcacac ctaccggcag gtggccccac
      721 ncctgcatta taannccaac agaa
//
