LOCUS       sequence-29915-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Geleziunas R, Ferrell S, Lin X, Mu Y, Cunningham ET, Grant M, 
            Connelly MA, Hambor JE, Marcu KB, Greene WC
  TITLE     Human T-cell leukemia virus type 1 Tax induction of NF-kappaB 
            involves activation of the IkappaB kinase alpha (IKKalpha) and 
            IKKbeta cellular kinases.
  JOURNAL   Mol Cell Biol. 1998 Sep . 18(9):5157-65.
  PUBMED    9710600
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Sep . 18(9):5157-65."
COMMENT     Sequence Label: pcDNA-Ikka-HA WT
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..71)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(51..77)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             350..358
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctagactcga gcggccgcca gtgtgatgga tcctcgaatt cctacaagct agcgtaatct
       61 ggaacatcgt atgggtattc tgctaaccaa ctccaatcaa gactcatcaa actactgctc
      121 tggtcctcat ttgcttcacg aataatagta cttaaatggc caagacagtt cagattttct
      181 tctatcattt gtgctaacgt ctctccatct tgaggagtta ccacacatgt cagaggatgt
      241 tcacggtctg ctaatgtagg gggcagccat gctgatactg gaggggttac tgtgccttct
      301 agactggatc ctacaagaga gcgggcagaa ctctgtgtac aggcaatttt aaggaggtgc
      361 caaatttctt tctgcctctt tccctgcata aacatgacag tattgtcagc ttctttgatg
      421 ttactgaggg ccacttccac cttggggagt agatcaataa tcttctgctt gcagcccaac
      481 aacttgctca ggtgaccaaa cagctccttg agaacacggt cctgactctg cacggtgtgc
      541 acgatgatct tcaccatctc tgtgctgtcg ctgtacaagt gatcaggagg tctgtgcttt
      601 agctgcttat agagatcaat ggcacgctgc tccagagact ccatcaagtc tccctggcgt
      661 cgtccgtagg ggctcttctg cagctccatg atttcagtgt gc
//
