LOCUS       sequence-29916-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Geleziunas R, Ferrell S, Lin X, Mu Y, Cunningham ET, Grant M, 
            Connelly MA, Hambor JE, Marcu KB, Greene WC
  TITLE     Human T-cell leukemia virus type 1 Tax induction of NF-kappaB 
            involves activation of the IkappaB kinase alpha (IKKalpha) and 
            IKKbeta cellular kinases.
  JOURNAL   Mol Cell Biol. 1998 Sep . 18(9):5157-65.
  PUBMED    9710600
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Sep . 18(9):5157-65."
COMMENT     Sequence Label: pcDNA-Ikka-HA (K44M)
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..78)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(58..84)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             357..365
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgggcctcta gactcgagcg gccgccagtg tgatggatcc tcgaattcct acaagctagc
       61 gtaatctgga acatcgtatg ggtattctgc taaccaactc caatcaagac tcatcaaact
      121 actgctctgg tcctcatttg cttcacgaat aatagtactt aaatggccaa gacagttcag
      181 attttcttct atcatttgtg ctaacgtctc tccatcttga ggagttacca cacatgtcag
      241 aggatgttca cggtctgcta atgtaggggg cagccatgct gatactggag gggttactgt
      301 gccttctaga ctggatccta caagagagcg ggcagaactc tgtgtacagg caattttaag
      361 gaggtgccaa atttctttct gcctctttcc ctgcataaac atgacagtat tgtcagcttc
      421 tttgatgtta ctgagggcca cttccacctt ggggagtaga tcaataatct tctgcttgca
      481 gcccaacaac ttgctcaggt gaccaaacag ctccttgaga acacggtcct gactctgcac
      541 ggtgtgcacg atgatcttca ccatctctgt gctgtcgctg tacaagtgat caggaggtct
      601 gtgctttagc tgcttataga gatcaatggc acgctgctcc agagactcca tcaagtctcc
      661 ctggcgtcgt ccgtaggggc tcttctgcag ctccatgatt tcagtgtgca aagacataat
      721 ttgatcctca agataaccaa tgacaccaac ctcagaatag tga
//
