LOCUS       sequence-29921-e         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Lin L, DeMartino GN, Greene WC
  TITLE     Cotranslational biogenesis of NF-kappaB p50 by the 26S proteasome.
  JOURNAL   Cell. 1998 Mar 20. 92(6):819-28.
  PUBMED    9529257
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1998 
            Mar 20. 92(6):819-28."
COMMENT     Sequence Label: pEV-p105-WT
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           78..117
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             153..185
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
       61 cgatccagcc tccgggggat cttggtggcg tgaaactccc gcacctcttc ggccagcgcc
      121 ttgtagaagc gcgtatggct tcgtggggat ctatggctag catgactggt ggacagcaaa
      181 tgggtcgcgg atccatggca gacgatgatc cctacggaac tgggcaaatg tttcatttga
      241 acactgcttt gactcactca atatttaatg cagaattata ttcaccagaa ataccactgt
      301 caacagatgg cccatacctt caaatattag agcaaccaaa acagagggga tttcgattcc
      361 gctatgtgtg tgaaggccca tcacacggag ggcttccggg agcctctagt gagaagaaca
      421 agaaatccta cccacaggtc aaaatttgca actatgtggg gcctgcaaag gttatcgttc
      481 agttggtcac aaatggaaaa aacatccacc tgcacgccca cagcctggtg ggcaagcact
      541 gtgaggacgg ggtatgcacc gtaacagcag gacccaagga catggtggtt ggctttgcaa
      601 acctgggaat acttcatgtg actaagaaaa angnatttga aacactg
//
