LOCUS       sequence-29922-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Chen LF, Williams SA, Mu Y, Nakano H, Duerr JM, Buckbinder L, Greene
            WC
  TITLE     NF-kappaB RelA phosphorylation regulates RelA acetylation.
  JOURNAL   Mol Cell Biol. 2005 Sep . 25(18):7966-75.
  PUBMED    16135789
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Sep . 25(18):7966-75."
COMMENT     Sequence Label: T7-RelA(S276A)
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           95..134
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             170..202
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc gggggatctt ggtggcgtga aactcccgca
      121 cctcttcggc cagcgccttg tagaagcgcg tatggcttcg tggggatcta tggctagcat
      181 gactggtgga cagcaaatgg gtcgcggatc cgacgaactg ttccccctca tcttcccggc
      241 agagccagcc caggcctctg gcccctatgt ggagatcatt gagcagccca agcagcgggg
      301 catgcgcttc cgctacaagt gcgaggggcg ctccgcgggc agcatcccag gcgagaggag
      361 cacagatacc accaagaccc accccaccat caagatcaat ggctacacag gaccagggac
      421 agtgcgcatc tccctggtca ccaaggaccc tcctcaccgg cctcaccccc acgagcttgt
      481 aggaaaggac tgccgggatg gcttctatga ggctgagctc tgcccggacc gctgcatcca
      541 cagtttccag aacctgggaa tccagtgtgt gaagaagcgg gacctggagc aggctatcag
      601 tcagcgcatc cagaccaaca acaacccctt ccaagttcct
//
