LOCUS       sequence-29924-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Chen LF, Williams SA, Mu Y, Nakano H, Duerr JM, Buckbinder L, Greene
            WC
  TITLE     NF-kappaB RelA phosphorylation regulates RelA acetylation.
  JOURNAL   Mol Cell Biol. 2005 Sep . 25(18):7966-75.
  PUBMED    16135789
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Sep . 25(18):7966-75."
COMMENT     Sequence Label: T7-RelA(S276E)
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           94..133
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             169..201
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg ggggatcttg gtggcgtgaa actcccgcac
      121 ctcttcggcc agcgccttgt agaagcgcgt atggcttcgt ggggatctat ggctagcatg
      181 actggtggac agcaaatggg tcgcggatcc gacgaactgt tccccctcat cttcccggca
      241 gagccagccc aggcctctgg cccctatgtg gagatcattg agcagcccaa gcagcggggc
      301 atgcgcttcc gctacaagtg cgaggggcgc tccgcgggca gcatcccagg cgagaggagc
      361 acagatacca ccaagaccca ccccaccatc aagatcaatg gctacacagg accagggaca
      421 gtgcgcatct ccctggtcac caaggaccct cctcaccggc ctcaccccca cgagcttgta
      481 ggaaaggact gccgggatgg cttctatgag gctgagctct gcccggaccg ctgcatccac
      541 agtttccaga acctgggaat ccagtgtgtg aagaagcggg acctggagca ggctatcagt
      601 cagcgcatcc agaccaacaa caaccccttc caagttcccn atagaaagag
//
