LOCUS       sequence-29928-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Auer S, Sturzebecher AS, Juttner R, Santos-Torres J, Hanack C, Frahm
            S, Liehl B, Ibanez-Tallon I
  TITLE     Silencing neurotransmission with membrane-tethered toxins.
  JOURNAL   Nat Methods. 2010 Mar;7(3):229-36. doi: 10.1038/nmeth.1425. Epub 
            2010 Feb 7.
  PUBMED    20139968
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1425"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "229-36"
COMMENT     Sequence Label: pFU-AgaIVA-VG
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             181..204
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(441..461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gagctgtaca agtccggccg gactcagatc ttaaagaaga agtgcatcgc caaggactac
       61 ggcaggtgca agtggggcgg caccccctgc tgtaggggca ggggctgcat ctgtagcatc
      121 atgggcacca actgcgagtg caagcccagg ctgatcatgg agggcctggg cctggccatc
      181 gattacaaag acgatgacga caagcttgcg gccgctggta acggaaatgg caacgggaat
      241 ggtaacggaa acggcaacgg ggatggtaat ggaggtgcac tctgcaacgg tgctggcttt
      301 gctaccccgg tgaccttggc cctggtccca gcactcctag ctaccttctg gagcttgctg
      361 taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
      421 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      481 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
      541 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
      601 actgtgtttg ctgacgcaac ccncactggt tggggcattg ccaccacc
//
