LOCUS       sequence-299285-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent labeling of the outer mitochondrial membrane (OMM) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abrisch RG, Gumbin SC, Wisniewski BT, Lackner LL, Voeltz GK
  TITLE     Fission and fusion machineries converge at ER contact sites to 
            regulate mitochondrial morphology.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 
            10.1083/jcb.201911122.
  PUBMED    32328629
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133843. doi"; 
            pages: " 10.1083/jcb.201911122"
COMMENT     Sequence Label: SNAP-OMP25
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..598
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggacaa
       61 agactgcgaa atgaagcgca ccaccctgga tagccctctg ggcaagctgg aactgtctgg
      121 gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc
      181 cgtggaagtg cctgccccag ccgccgtgct gggcggacca gagccactga tgcaggccac
      241 cgcctggctc aacgcctact ttcaccagcc tgaggccatc gaggagttcc ctgtgccagc
      301 cctgcaccac ccagtgttcc agcaggagag ctttacccgc caggtgctgt ggaaactgct
      361 gaaagtggtg aagttcggag aggtcatcag ctacagccac ctggccgccc tggccggcaa
      421 tcccgccgcc accgccgccg tgaaaaccgc cctgagcgga aatcccgtgc ccattctgat
      481 cccctgccac cgggtggtgc agggcgacct ggacgtgggg ggctacgagg gcgggctcgc
      541 cgtgaaagag tggctgctgg cccacgaggg ccacagactg ggcaagcctg ggctgggtag
      601 cggtagcggt ggcagctctc gaggtacgcg tgggcgaggc gacggagagc cgagtggagt
      661 tcctgtagct gtggtgctgc tgccagtgtt tgcccttacc ctggtagcag tttgggcctt
      721 cgtgagatac cgaaagcagc tctgangatc caccggatct agataactga tcataatcag
      781 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      841 cctgaaacat aaaatgaatg caattgttgt tgttaacttg nttnntgcag cttataatgg
      901 ntacaaataa agcatagcat cacaaatttc ncnaatnaag cntttttnna ctgcatncta
      961 nttgnnngnt nnnccnaact catcaangna tcttaacgcg taaanngtaa gcgnnnnnnt
     1021 nnnnaannnc gnnnannntn gnnaancagc tcntttttnn nantngnnaa ncggcaaatc
     1081 cnnnnnnann a
//
