LOCUS       sequence-299286-        2487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0071
FEATURES             Location/Qualifiers
     source          1..2487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     474..493
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     721..739
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(743..786)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        883..1228
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1171..1191
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1249..1306
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1330..1347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1501..2089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1581..1600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2272..2366)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2387..2487,1..559))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
       61 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
      121 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
      181 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
      241 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
      301 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
      361 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
      421 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
      481 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
      541 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
      601 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
      661 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      721 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
      781 gatttctgga attcggtctc aggaggtcaa catggtggag cacgacactc tggtctactc
      841 caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt ttcaacaaag
      901 gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
      961 gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat
     1021 cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     1081 cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat gtgacatctc
     1141 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     1201 aggaagttca tttcatttgg agaggacacg ctactcgaga ccctgcagtc cggcaaaaaa
     1261 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     1321 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1501 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2221 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc
     2281 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     2341 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     2401 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     2461 aaacggcatg atgaacctga atcgcca
//
