LOCUS       sequence-299287-        2519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0095
FEATURES             Location/Qualifiers
     source          1..2519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     496..515
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     743..761
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(765..808)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1303..1360
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1384..1401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1555..2143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1635..1654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2326..2420)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2441..2519,1..581))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
       61 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      121 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
      181 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      241 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
      301 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
      361 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
      421 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
      481 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
      541 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
      601 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
      661 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
      721 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
      781 atccttagct ttcgctaagg atgatttctg gaattcggtc tcaggagtgg aagattcaga
      841 aaatctccat caacaaataa tccaagtaag gattaatgga ttgatcaaca tccttaccgc
      901 tatgggtaag attgatgaaa agtcaaaaac aaaaatcaat tatgcacacc agcatgtgtt
      961 gatcaccagc tattgtggga caccaatttc gtccacagac atcaacatct tatcgtcctt
     1021 tgaagataag ataataatgt tgaagataag agtgggagcc accactaaaa cattgctttg
     1081 tcaaaagcta aaaaagatga tgcccgacag ccacttgtgt gaagcatgtg aagccggtcc
     1141 ctccactaag aaaattagtg aagcatcttc cagtggtccc tccactcaca gctcaatcag
     1201 tgagcaacag gacgaaggaa atgacgtaag ccatgacgtc taatcccaca agaatttcct
     1261 tatataagga acacaaatca gaaggtactc gagaccctgc agtccggcaa aaaagggcaa
     1321 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
     1381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1561 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2281 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
     2341 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     2401 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     2461 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacg
//
