LOCUS       sequence-299290-        2519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0099
FEATURES             Location/Qualifiers
     source          1..2519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     488..507
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     735..753
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(757..800)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1295..1352
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1376..1393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1547..2135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1627..1646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2318..2412)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2433..2519,1..573))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
       61 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      121 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
      181 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      241 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
      301 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
      361 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
      421 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
      481 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
      541 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
      601 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
      661 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
      721 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
      781 ctttcgctaa ggatgatttc tggaattcgg tctcaggagt ggaagattca gaaaatctcc
      841 atcaacaaat aatccaagta aggattaatg gattgatcaa catccttacc gctatgggta
      901 agattgatga aaagtcaaaa acaaaaatca attatgcaca ccagcatgtg ttgatcacca
      961 gctattgtgg gacaccaatt tcgtccacag acatcaacat cttatcgtcc tttgaagata
     1021 agataataat gttgaagata agaatgacgt aagccatgac gtctactaaa acattgtggg
     1081 agccaccacc gacagccact tgtgtgaagc atgtgaagcc ggtccctcca ctaagaaaat
     1141 tagtgaagca tcttccagtg gtccctccac tcacagctca atcagtgagc aacaggacga
     1201 aggaagcttt gtcaaaagct aaaaaagatg atgcatccca caagaatttc cttatataag
     1261 gaacacaaat cagaaggtac tcgagaccct gcagtccggc aaaaaagggc aaggtgtcac
     1321 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
     1381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1981 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2161 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2221 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2281 tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc
     2341 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     2401 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     2461 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatg
//
