LOCUS       sequence-299297-        2467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0279
FEATURES             Location/Qualifiers
     source          1..2467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     250..269
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     497..515
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(519..562)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     927..947
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        936..982
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1005..1062
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1086..1103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1257..1845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1337..1356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2028..2122)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2143..2467,1..335))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
       61 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      121 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      181 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      241 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      301 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
      361 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      421 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
      481 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
      541 agctttcgct aaggatgatt tctggaattc ggtctcagga ggtcaacatg gtggagcacg
      601 acactctggt ctactccaaa aatgtcaaag atacagtctc agaagatcaa agggctattg
      661 agacttttca acaaaggatg attccattgc ccagctatct gtcacttcat cgaaaggaca
      721 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
      781 caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      841 gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga catctccact
      901 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      961 agttcatttc atttggagag gacacgctac tcgagaccct gcagtccggc aaaaaagggc
     1021 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag
     1081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1261 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1981 atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata
     2041 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     2101 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     2161 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     2221 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     2281 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     2341 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     2401 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     2461 ccggaaa
//
