LOCUS       sequence-299298-        2469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0277
FEATURES             Location/Qualifiers
     source          1..2469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     270..289
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     517..535
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(539..582)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     949..969
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        958..1004
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1027..1084
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1108..1125)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1279..1867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1359..1378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2050..2144)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2165..2469,1..355))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
       61 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      121 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      181 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      241 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      301 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      361 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      421 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
      481 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
      541 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga
      601 ggtcaacatg gtggagcacg acactctggt ctactccaaa aatgtcaaag atacagtctc
      661 agaagatcaa agggctattg agacttttca acaaaggata atttcgggaa acctcctcgg
      721 attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aaggtggctc
      781 ctacaaatgc catcctatca ttcaagatct ctctgccgac agtggtccca aagatggacc
      841 cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt
      901 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      961 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct actcgagacc
     1021 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     1081 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1261 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     1321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1681 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1921 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1981 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     2041 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     2101 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     2161 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     2221 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2281 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2341 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     2401 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2461 gcgaatata
//
