LOCUS       sequence-29930-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  TITLE     Rimm Plasmids
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Bcatfl
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             152..187
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 tggcttagcg agctctctgg ctactgagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagcttgg taccgagctc ggatccatgg ctactcaagc
      121 tgatttgatg gagttggaca tggccatgga accagacaga aaagcggctg ttagtcactg
      181 gcagcaacag tcttacctgg actctggaat ccattctggt gccactacca cagctccttc
      241 tctgagtggt aaaggcaatc ctgaggaaga ggatgtggat acctcccaag tcctgtatga
      301 gtgggaacag ggattttctc agtccttcac tcaagaacaa gtagctgata ttgatggaca
      361 gtatgcaatg actcgagctc agagggtacg agctgctatg ttccctgaga cattagatga
      421 gggcatgcag atcccatcta cacagtttga tgctgctcat cccactaatg tccagcgttt
      481 ggctgaacca tcacagatgc tgaaacatgc agttgtaaac ttgattaact atcaagatga
      541 tgcagaactt gccacacgtg caatccctga actgacaaaa ctgctaaatg acgaggacca
      601 ggtggtggtt aataaggctg cagttatggt ccatcagctt tctaaaaagg aagcttccag
      661 acacgctatc atgcgttctc ctcagatggt gtctgctatt gtacgtacca tgcagaatac
      721 aaatgatgta gaacagctcg ttgtaccgct gggacttgca taacctttcc atcatcgtga
      781 ggctactggc atcttantct ga
//
