LOCUS       sequence-299303-        2506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0288
FEATURES             Location/Qualifiers
     source          1..2506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     286..305
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     533..551
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(555..598)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1080..1137
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1161..1178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1332..1920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1412..1431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2103..2197)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2218..2506,1..371))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
       61 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
      121 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
      181 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
      241 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
      301 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
      361 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
      421 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg
      481 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      541 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
      601 gaattcggtc tcaggagtgg aagattcaga aaatctccat caacaaataa tccaagtaag
      661 gattaatgga ttgatcaaca tccttaccgc tatgggtaag attgatgaaa agtcaaaaac
      721 aaaaatcaat tatgcacacc agcatgtgtt gatcaccagc tattgtggga caccaatttc
      781 gtccacagac atcaacatct tatcgtcctt tgaagataag ataataatgt tgaagataag
      841 agtgggagcc accactaaaa cattgctttg tcaaaagcta aaaaagatga tgcccgacag
      901 ccacttgtgt gaagcatgtg aagccggtcc ctccactaag aaaattagag tggtccctcc
      961 actcacagct caatcagtga gcaacaggac gaaggaaatg acgtaagcca tgacgtctaa
     1021 tcccacaaga atttccttat ataaggaaca caaatcagaa ggtactcgag accctgcagt
     1081 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
     1141 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1201 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1261 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1321 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1741 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     2101 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2161 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     2221 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     2281 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2341 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2401 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     2461 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgcc
//
