LOCUS       sequence-299308-        2483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0291
FEATURES             Location/Qualifiers
     source          1..2483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     557..575
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(579..622)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1081..1138
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1162..1179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1333..1921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1413..1432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2104..2198)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2219..2483,1..395))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
       61 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      121 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      181 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      241 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      301 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      361 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
      421 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      481 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
      541 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
      601 agctttcgct aaggatgatt tctggaattc ggtctcagga gtggaagatt cagaaaatct
      661 ccatcaacaa ataatccaag taaggattaa tggattgatc aacatcctta ccgctatggg
      721 tgcacaccag catgtgttga tcaccagcta ttgtgggaca ccaatttcgt ccacagacat
      781 caacatctta tcgtcctttg aagataagat aataatgttg aagataagag tgggagccac
      841 cactaaaaca ttgctttgtc aaaagctaaa aaagatgatg cccgacagcc acttgtgtga
      901 agcatgtgaa gccggtccct ccactaagaa aattagtgaa gcatcttcca gtggtccctc
      961 cactcacagc tcaatcagtg agcaacagga cgaaggaaat gacgtaagcc atgacgtcta
     1021 atcccacaag aatttcctta tataaggaac acaaatcaga aggtactcga gaccctgcag
     1081 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     1141 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1201 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1261 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1321 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1381 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1441 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1501 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1561 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1621 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1681 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1741 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1801 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1861 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1921 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1981 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2041 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
     2101 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     2161 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     2221 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2281 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2341 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2401 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2461 caataaaccc tttagggaaa tag
//
