LOCUS       sequence-299322-        9412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SARS-CoV-2 nsp12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P
  TITLE     Structure of replicating SARS-CoV-2 polymerase.
  JOURNAL   Nature. 2020 May 21. pii: 10.1038/s41586-020-2368-8. doi: 
            10.1038/s41586-020-2368-8.
  PUBMED    32438371
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 May 21. pii: 10.1038/s41586-020-2368-8. doi: 
            10.1038/s41586-020-2368-8."
COMMENT     Sequence Label: pFastBac-His-MBP-SCov2-nsp12
FEATURES             Location/Qualifiers
     source          1..9412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  68..292
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(359..892)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1062..1067,1068..1084,1085..1090))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     1499..1516
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1636..1653
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1663..2763
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2737..2760
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2818..2838
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    5870..6004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5907..5926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5961..5980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6078..6243
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6427..6882
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6514..6533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6724..6745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6909..7013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7014..7082,7083..7874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7232..7251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8045..8633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8534..8553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8787..8804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8888..8907)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9048..9070
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9064..9255)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gaccagccgc gtaacctggc aaaatcggtt acggttgagt aataaatgga tgccctgcgt
       61 aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca
      121 ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
      181 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
      241 gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta aagactatat tcgcggcgtt
      301 gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt
      361 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
      421 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
      481 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
      541 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
      601 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
      661 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
      721 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
      781 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
      841 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc
      901 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
      961 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     1021 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
     1081 cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     1141 tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg
     1201 tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca
     1261 cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg
     1321 tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt
     1381 tcgcccagga ctctagctat agttctagtg gttggctacg tatactccgg actattaata
     1441 gatcatggag ataattagcg gccgcgttta aacatccacc atttgatgga gataattaaa
     1501 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     1561 aaaaacctat aactattccg gattattcat accgtcccac catcgggcgc ggatcccgga
     1621 ccgatgggtt cttctcacca tcaccatcac catggttctt ctatgaaaat cgaagaaggt
     1681 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
     1741 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
     1801 aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
     1861 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
     1921 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
     1981 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
     2041 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
     2101 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
     2161 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
     2221 gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
     2281 gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
     2341 atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
     2401 gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
     2461 attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg
     2521 actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
     2581 tcttacgagg aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag
     2641 aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
     2701 gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
     2761 actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggaa
     2821 aacctgtact tccaatccaa tgcatctgct gacgctcagt ccttcctgaa ccgtgtgtgc
     2881 ggtgtctctg ctgctcgtct gactccttgc ggtactggaa cctctaccga cgtggtgtac
     2941 cgtgctttcg acatctacaa cgacaaggtg gccggtttcg ctaagttcct caagaccaac
     3001 tgctgccgct tccaagagaa ggacgaggac gacaacctga tcgactccta cttcgtggtc
     3061 aagcgtcaca ccttctccaa ctaccagcac gaggaaacta tctacaacct gctgaaggac
     3121 tgccccgctg tggctaagca cgacttcttc aagttccgta tcgacggcga catggtgccc
     3181 cacatctctc gtcagcgtct gaccaagtac accatggctg acctggtgta cgctctgcgc
     3241 cacttcgatg agggaaactg cgacaccctg aaagagatcc tggtcaccta caactgctgt
     3301 gacgacgact acttcaacaa gaaggattgg tacgacttcg tcgagaaccc cgacatcctg
     3361 cgtgtgtacg ctaacctggg agagcgtgtg cgtcaggctc tgctcaagac tgtgcagttc
     3421 tgcgacgcta tgcgtaacgc tggtatcgtg ggagtgctga ccctggacaa ccaggacctg
     3481 aacggcaatt ggtatgattt cggcgacttc atccagacta cccctggttc tggtgtcccc
     3541 gtggtggact cttactactc cctgctgatg cccatcctga ctctgacccg tgctctgacc
     3601 gctgagtctc acgtggacac cgacctgacc aagccttaca tcaagtggga cttgctgaag
     3661 tacgatttca ccgaggaacg cctgaagctg ttcgaccgtt acttcaagta ctgggaccag
     3721 acctaccatc ctaactgcgt gaactgcctg gacgaccgtt gcatcctgca ctgcgctaac
     3781 ttcaacgtgc tgttctccac tgtgttccct ccaacctcct tcggtcctct cgtgcgcaag
     3841 atcttcgtgg acggtgtccc tttcgtggtg tccaccggtt accacttccg tgaactgggt
     3901 gtcgtgcata accaggacgt caacctccac tcctctcgtc tgtccttcaa agagctgctg
     3961 gtctacgctg ctgaccccgc tatgcatgct gcttccggaa acctgctgct ggacaagcgt
     4021 accacttgct tctccgtggc tgccctgact aacaacgtgg ccttccagac cgtgaagccc
     4081 ggcaatttca acaaggactt ctacgatttc gccgtgtcca agggcttctt caaagaaggc
     4141 tcctccgtcg agctgaagca cttcttcttc gctcaagacg gcaacgctgc tatctccgac
     4201 tacgactact accgttacaa cttgcccacc atgtgcgaca tccgtcagct gctgttcgtg
     4261 gtcgaggtgg tggacaagta cttcgactgc tacgacggtg gttgcatcaa cgccaatcaa
     4321 gtgatcgtga acaacctgga caagtccgct ggcttcccct tcaacaaatg gggcaaagct
     4381 cgtctgtact acgactccat gtcctacgag gaccaggacg ctctgttcgc ttacaccaag
     4441 cgtaacgtga tccccaccat cactcagatg aacctgaaat acgccatctc cgctaagaac
     4501 cgtgctcgta ccgtggctgg tgtctccatc tgctccacca tgaccaaccg tcagttccac
     4561 cagaagctgc tgaagtcaat cgctgctacc cgcggtgcta ccgtggtcat cggtacttcc
     4621 aagttctacg gtggctggca caacatgctg aaaaccgtgt actccgacgt ggaaaaccct
     4681 cacctgatgg gctgggacta ccccaagtgc gatcgtgcta tgcctaacat gctgcgtatc
     4741 atggcttccc tggtgctggc tcgcaagcac actacttgct gctccctgtc tcaccgtttc
     4801 taccgtctgg ctaacgagtg cgctcaggtg ctgtccgaga tggtcatgtg cggtggttcc
     4861 ctgtacgtga agcctggtgg tacttcctct ggcgacgcta ccaccgctta cgctaactcc
     4921 gtgttcaaca tctgccaggc tgtgaccgct aacgtgaacg ctctgctgtc caccgacggc
     4981 aacaagatcg ctgacaaata cgtgcgtaac ctgcagcacc gtctgtacga gtgcctgtac
     5041 cgtaaccgtg acgtcgacac tgacttcgtg aacgagttct acgcttacct gcgcaagcac
     5101 ttcagcatga tgatcctgtc cgacgacgct gtcgtgtgct tcaactccac ctacgcttcc
     5161 caaggcctgg tggcttccat caagaacttc aagtccgtgc tgtactacca gaacaacgtg
     5221 ttcatgtccg aggctaagtg ctggaccgag actgacctga ctaagggtcc ccacgagttc
     5281 tgctcccagc acaccatgct ggtcaagcag ggcgacgatt acgtgtacct gccttatcct
     5341 gatccttctc gtatcctcgg cgctggttgc ttcgtggatg acatcgtcaa gaccgacgga
     5401 accctgatga tcgagcgttt cgtgtccctg gctatcgacg cttaccctct gactaagcac
     5461 cccaatcaag agtacgctga cgtgttccac ctgtacctgc agtacatccg caagctgcac
     5521 gacgagctga ccggacacat gctggacatg tactccgtga tgctgaccaa cgacaacacc
     5581 tctcgttact gggagcccga attctacgag gctatgtaca cccctcacac cgtgctgcaa
     5641 taataacatt ggaagtggat aacggatccg aattcgagcg ccgtcgacaa gcttgcggcc
     5701 tcatgatctc ctaggggccg cactcgaggc atgcggtacc aagcttgtcg agaagtacta
     5761 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6001 gatctgatca ctgcttgagc ctagaaacat ccaccattta aatggagtta ggtttaaacg
     6061 cctgcagggg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     6121 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     6181 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     6241 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
     6301 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
     6361 cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg
     6421 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6481 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6541 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6601 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6661 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6721 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6781 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6841 aatttaacgc gaattttaac aaactattaa cgtttacaat ttcaggtggc acttttcggg
     6901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6961 tcatgagaca ataaccctga taaatgcttc aatactattg aaaaaggaag agtatgagta
     7021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagaggcgc
     8821 gccatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca
     8881 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     8941 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     9001 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9061 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     9121 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     9181 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     9241 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     9301 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     9361 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc ag
//
