LOCUS       sequence-299326-        8678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses WDR92.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7134 pHAGE-P-CMVt-N-HA-GAW-WDR92
FEATURES             Location/Qualifiers
     source          1..8678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    23..140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        192..495
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        496..699
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     650..670
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    701..719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    722..740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     750..774
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      861..870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             873..899
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    915..939
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1804..1828)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1847..2346
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1882..1905)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2279..2298
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2367..2966
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2367..2386)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2863..2883
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3033..3621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3086..3106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3504..3515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3696..3929
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4048..4066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4134..4238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4239..4307,4308..5099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4457..4476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5270..5858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5759..5778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6012..6029
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6269..6290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6305..6310,6311..6328,6329..6335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6343..6359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6348..6370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6367..6383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6367..6383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6433..6452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6644..7277
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7324..7449
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7946..8179
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8364..8408
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
       61 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      121 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta
      181 gtggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      241 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      301 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      361 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      421 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      481 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      541 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      601 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      661 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
      721 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
      781 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      841 actctagcgt ttaaacgcta gccaccatgg cgtacccata cgatgttcca gattacgctg
      901 ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcat gtcggccttc gagaagcctc
      961 agatcatcgc ccatatccag aagggcttca actacacggt gtttgactgt aagtgggtgc
     1021 cctgcagcgc caaatttgtg accatgggca acttcgcacg gggcaccggc gtcattcagc
     1081 tgtacgagat ccagcacggg gacctgaagc tgcttcggga gattgaaaag gccaaaccta
     1141 ttaaatgtgg aacatttggt gcaacatctt tacagcagag atatttagct actggagatt
     1201 ttggtggaaa ccttcatata tggaatttag aagctccaga gatgccagta tattctgtaa
     1261 agggccataa agaaattata aatgccatag atggcatagg tggactagga attggagaag
     1321 gagcacctga aattgtgact ggcagccgag atggaactgt gaaggtgtgg gacccaaggc
     1381 aaaaagatga tcctgttgct aatatggaac ctgtacaagg agaaaacaag agagactgtt
     1441 ggactgtggc atttggcaat gcttataatc aagaagaacg tgttgtttgt gctggctatg
     1501 acaatgggga tatcaaacta tttgatctca gaaatatggc attacggtgg gagacaaaca
     1561 tcaaaaatgg ggtgtgtagc ttggagtttg acagaaaaga cataagtatg aataagttag
     1621 tagccacatc tctggaagga aagttccatg tttttgacat gagaacacag catccaacca
     1681 aaggttttgc ctctgtttca gaaaaggctc ataaatctac tgtgtggcag gtccgacacc
     1741 tgccgcagaa cagggagctc tttctgacag ctggaggcgc cggcggcctt cacctctgga
     1801 agtacccaac tttcttgtac aaagtggttc gataacgaat tctaccgggt aggggaggcg
     1861 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     1921 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     1981 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     2041 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     2101 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     2161 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     2221 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     2281 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     2341 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     2401 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     2461 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     2521 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     2581 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     2641 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     2701 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     2761 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     2821 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     2881 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     2941 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     3001 ggagcgcacg accccatgca tcgatagatc ctaatcaacc tctggattac aaaatttgtg
     3061 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3121 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3181 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3241 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3301 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3361 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3421 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     3481 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     3541 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3601 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt
     3661 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3721 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     3781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     3841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3901 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     3961 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag
     4021 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4081 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4261 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4321 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4381 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4441 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     4501 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4561 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4621 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4681 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4741 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4801 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4861 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4921 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4981 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5041 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5101 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5161 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5221 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5281 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5341 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5401 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5461 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5521 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5581 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5641 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5701 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5761 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5821 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5881 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5941 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6001 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6061 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt
     6121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6181 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
     6241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6361 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
     6421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     6481 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     6541 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     6601 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
     6661 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     6721 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     6781 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     6841 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     6901 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     6961 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     7021 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     7081 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7141 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7201 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7261 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7321 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7381 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7441 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7501 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7561 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     7621 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7681 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7741 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     7801 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     7861 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     7921 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     7981 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     8041 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     8101 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     8161 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     8221 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     8281 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     8341 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     8401 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     8461 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     8521 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     8581 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     8641 gattagtgaa cggatctcga cggtatcgcc gaattcac
//
