LOCUS       sequence-299339-       11084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Kalirin-7 with the b-front in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Miller MB, Yan Y, Wu Y, Hao B, Mains RE, Eipper BA
  TITLE     Alternate promoter usage generates two subpopulations of the 
            neuronal RhoGEF Kalirin-7.
  JOURNAL   J Neurochem. 2017 Mar;140(6):889-902. doi: 10.1111/jnc.13749. Epub 
            2016 Sep 6.
  PUBMED    27465683
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/jnc.13749";
            journalName: "J Neurochem"; date: "2017-03"; volume: "140"; issue: 
            "6"; pages: "889-902"
COMMENT     Sequence Label: pEAK-bKal7
FEATURES             Location/Qualifiers
     source          1..11084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          897..1100
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     1057..1077
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1117..1126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6455..6589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6492..6511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6546..6565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(6594..7193)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6677..6697)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7174..7193
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     rep_origin      9211..9346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9841..9860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(10029..10097,10098..10889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDHLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10247..10266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      complement(10887..11022)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga cgatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
      601 cccgtgcgtc ccagcgcaca tgcatgttcg gcgaggcggg gcctgcgagc gcggccaccg
      661 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
      721 ccgtgtatcg ccccgccctg ggcggcaagg ctgggagctc aaaatggagg acgcggcgct
      781 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
      841 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
      901 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
      961 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1021 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1081 gtttttttct tccatttcag gtgtcgtgaa aagcttgcca ccatgggaaa ccccccggag
     1141 ggggcatcgg aggaaggggg agccgccgac tcggacgtgg acgccttttt ccggacagga
     1201 tcttttcgga atgatggttt gaaagcttct gatgtccttc ctatcctaaa ggagaaagtg
     1261 gcctttgtgt ctgggggtcg cgataagcga ggtggaccca tcctgacctt ccctgcccga
     1321 agcaatcatg acagaataag acaggaagac ctacggaaac tcgtgacgta tttggccagc
     1381 gtgccaagcg aggacgtttg caaacgtggc ttcaccgtca tcatcgacat gcggggctcc
     1441 aagtgggacc tcatcaagcc cctcctcaag acgctgcagg aagcctttcc cgccgagatc
     1501 catgtggccc tcatcatcaa gcctgacaac ttctggcaga agcagaagac caactttggc
     1561 agctccaaat tcatctttga gacgagcatg gtgtctgtgg agggcctcac gaagctggtg
     1621 gacccctccc agctgacaga agagtttgac ggttccctgg attacaacca cgaggagtgg
     1681 attgagttgc gcctctccct ggaggagttc ttcaacagtg cggtccacct gctctcacgc
     1741 ctcgaggacc tgcaggagat gctggcccgg aaggagttcc ctgtggatgt ggagggctct
     1801 cggaggctta tcgacgagca cacacaactt aagaaaaagg tgctgaaggc ccccgtggaa
     1861 gagctggacc gggaggggca gcggctactg cagtgtatcc gctgcagtga tggcttctca
     1921 gggcgcaact gcatcccagg aagtgccgac ttccagagcc ttgtacccaa gatcaccagc
     1981 ctcctggaca agctgcattc cacccgacaa cacctgcacc agatgtggca cgtccgaaag
     2041 ctcaagctgg atcagtgctt ccagctgcgg ctcttcgagc aggatgccga gaagatgttc
     2101 gactggataa gccacaacaa ggagttattt cttcagagcc acacagagat cggagtcagc
     2161 taccagcatg cactagacct ccagacacag cacaatcact ttgccatgaa ctccatgaat
     2221 gcctatgtca acatcaaccg catcatgtct gtcgcatccc gcctctcaga ggctggccat
     2281 tatgcctccc agcaaatcaa gcagatctcc acgcagctag accaggagtg gaagagcttt
     2341 gctgctgccc tggatgaacg cagtacaatc ctggccatgt ctgctgtgtt ccaccagaag
     2401 gcagagcagt tcctgtcagg agtggatgcc tggtgtaaga tgtgcagtga aggcggcctt
     2461 ccttctgaga tgcaggacct ggagctggca atccaccatc accagtcctt gtatgagcag
     2521 gtgacccagg cctacacaga ggtcagccag gatgggaaag cactcctgga tgtcctgcag
     2581 cgtcccctga gccctgggaa ctccgagtcc ctcacagcca cagccaacta ctccaaggcg
     2641 gtacaccagg tgctggatgt ggtacatgag gtgctgcacc accagcggcg gctcgagagc
     2701 atctggcagc accgtaaagt gcggcttcac cagcggctgc agctgtgcgt cttccagcag
     2761 gatgtgcagc aggtgctgga ctggattgaa aaccacggtg aggcctttct cagcaaacac
     2821 actggggtcg gcaagtccct gcatcgggca cgggcattgc agaagaggca tgatgacttt
     2881 gaagaggtgg ctcagaatac atacaccaat gcggacaagc tcctagaggc agcagagcag
     2941 ctggctcaga ctggggaatg tgacccggaa gagatctaca aggctgcccg acacctggag
     3001 gtgcgcatcc aagacttcgt gcgcagagtg gagcagcgga agcttcttct ggacatgtcc
     3061 gtctctttcc acacgcacac caaagagctg tggacatgga tggaagacct tcagaaggag
     3121 gtgctggagg atgtctgtgc agactctgtg gatgcggtcc aggaactgat caagcagttc
     3181 cagcagcagc agaccgctac cctggacgcc actctcaacg tcatcaagga aggcgaagac
     3241 cttatccagc agctcaggtc agcgcctccc tccctggggg agcccaccga ggccagggac
     3301 tcagctgtat ccaacaacaa gacgccccac agtagttcca tcagccatat cgagtctgtc
     3361 ctccagcagc tggacgatgc tcaggtacag atggaggagc tgtttcacga gcggaagatc
     3421 aagctggaca tcttcctgca gctgcgcatc ttcgaacagt acaccattga ggtcaccgca
     3481 gagctggatg cctggaatga ggacttgctc cggcagatga atgacttcaa cacagaggac
     3541 ctgaccctgg cagagcagcg gctacagcgc cacacagaac ggaagcttgc catgaacaac
     3601 atgacattcg aggtcatcca gcagggccag gacctgcacc aatatatcat ggaagtccaa
     3661 gcctcaggaa tcgagttaat ctgtgagaag gacgttgacc tggcagctca ggtgcaagag
     3721 ctactggaat ttctccatga gaagcagcac gaactggagc tcaatgcgga gcagacgcac
     3781 aagcggctag agcagtgtct ccagctgcgg cacctccagg ctgaggtcaa acaggttctg
     3841 ggatggatcc gcaatggaga gtccatgctc aacgccagcc tggtcaatgc cagctctttg
     3901 tctgaggcag agcagctaca gcgggagcac gaacagttcc agctagccat cgagaagacg
     3961 caccagagcg ccctgcaggt acagcagaag gcagaggcac tgctccaggc cggccactat
     4021 gacgccgatg ctatccggga atgtgcggag aaggtggccc tccactggca acagctcatg
     4081 ctaaagatgg aggaccggct gaaactcgtc aatgcctctg tggcctttta caagacctct
     4141 gagcaggtgt gcagcgttct ggagagctta gagcaggagt accggagaga cgaagactgg
     4201 tgcggcggga gagacaagct ggggccggcg gcagagatgg accacgtcat tccactcctc
     4261 agcaagcatc tggagcagaa ggaggccttc ctgaaggcct gcactctggc acggcggaat
     4321 gccgaggtgt tcctcaagta catccacagg aacaatgtga gcatgcccag tgttgccagc
     4381 cacaccaggg gacccgaaca gcaagtaaaa gccatcttga gtgagctctt gcagagagag
     4441 aatcgcgtcc tgcacttctg gaccttgaag aagcggcggt tagaccagtg tcagcaatat
     4501 gtggtgtttg aacgcagtgc taaacaggca ctagactgga tccaagaaac aggagaatat
     4561 tacctctcaa cccatacctc cactggagag accacagagg agactcagga gctgctgaaa
     4621 gagtatggag aattcagggt gcctgccaag caaacaaaag agaaggtgaa gcttctgatc
     4681 cagctagcag acagctttgt ggaaaagggc cacatccatg ccacagagat acggaaatgg
     4741 gtgaccacgg tggacaagca ctaccgggat ttctccctga ggatggggaa gtaccggtac
     4801 accctggaga aagccctggg agtcaacacg gaggataaca aggacctaga gctggacatt
     4861 atcccagcaa gcctttcaga ccgggaggtc aagctccggg atgctaacca cgaggtcaat
     4921 gaagagaaac ggaagtcagc tcgaaagaaa gaattcatta tggctgaact gctccagacc
     4981 gagaaggctt atgtaagaga cctacacgag tgcttggaga cctacctgtg ggaaatgacc
     5041 agtggtgtgg aagagatacc acctgggatc ctcaataagg agcacatcat cttcggcaac
     5101 atccaggaga tctatgactt ccacaacaac atcttcctca aagagctgga gaagtacgag
     5161 cagctgcctg aggatgtggg acactgcttt gttacctggg cagacaaatt tcagatgtat
     5221 gtcacctact gtaaaaacaa gcctgattcc aaccagctga tcctggagca cgcaggcacc
     5281 ttctttgatg agatccagca gcggcacggg ctggccaact ccatctcttc ctacttaatt
     5341 aagcctgtcc agagagttac gaagtaccag ctgctcctga aggaactttt aacttgctgt
     5401 gaagaaggga aaggggagct caaggatggc ctggaggtga tgctcagtgt ccccaagaaa
     5461 gccaatgatg ccatgcatgt cagcatgctt gaagggttcg atgagaactt ggatgtacag
     5521 ggggagttga ttctccagga tgcctttcaa gtgtgggacc caaagtcgct gatccggaag
     5581 gggcgggaac ggcatctgtt cctctttgag atctccttgg tgtttagcaa agagatcaaa
     5641 gactcttcag gacacacgaa atatgtttac aagaacaagc tactgacctc agagctgggt
     5701 gtgaccgagc acgtagaagg tgacccctgc aaattcgcct tgtggtctgg gcgcacccca
     5761 tcctcagaca ataaaacagt gctcaaagct tccaacatcg aaaccaagca ggaatggatc
     5821 aagaacattc gggaagtgat tcaggaaagg atcattcatc ttaaaggagc tttaaaggag
     5881 ccaatccagc tccccaaaac accagccaaa ctgaggaaca atagtaagag ggatggggta
     5941 gaggatggtg acagccaggg ggacgggagc agccagcccg ataccatatc cattgcctcc
     6001 aggacctctc agaacacagt ggagagtgac aaggatggca accttgttcc tcggtggcac
     6061 ctgggacctg gagatccttt ctccacttac gtttagcgcg cagcctggga cttgtccctg
     6121 cagctcaccg ggttagccgt ctagagcggc cgcaggtaag ccagcccagg cctcgccctc
     6181 cagctcaagg cgggacaggt gccctagagt agcctgcatc cagggacagg ccccagccgg
     6241 gtgctgacac gtccacctcc atctcttcct caggtctgcc cgggtggcat ccctgtgacc
     6301 cctccccagt gcctctcctg gtcgtggaag gtgctactcc agtgcccacc agccttgtcc
     6361 taataaaatt aagttgcatc attttgtttg actaggtgtc cttgtataat attatggggt
     6421 ggaggcgggt ggtatggagc aaggggccca agttaacttg tttattgcag cttataatgg
     6481 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6541 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gcttcaggca
     6601 ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg
     6661 acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag
     6721 gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga
     6781 cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg
     6841 ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagcct ggaaccgctc
     6901 aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc
     6961 gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc
     7021 ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg
     7081 tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc
     7141 cgggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtggcc
     7201 tgcagagtcg ctctgtgttc gaggccacac gcgtcacctt aatatgcgaa gtggacctgg
     7261 gaccgcgccg ccccgactgc atctgcgtgt tttcgccaat gacaagacgc tgggcggggt
     7321 ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca ccgccagcaa
     7381 acgcgagcaa cgggccacgg ggatgaagca gctgcgccac tccctgaaga tccatcgtct
     7441 cctaacaagt tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct
     7501 ccgtcatctc cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga
     7561 ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg
     7621 ggctttgtca taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt
     7681 tgtaaaaaga ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg
     7741 ggtccagggg ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata
     7801 gcaagggcag ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac
     7861 acaccggcga cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga
     7921 gctcttaaac cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc
     7981 tttccaaacc accctccttt tttgcgcctg cctccatcac cctgaccccc gctgcgcggg
     8041 ggcacgtcag gctcaccatc tgggccgcct tcttggtggt attcaaaata atcggcttcc
     8101 cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat
     8161 gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc
     8221 ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca
     8281 ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga
     8341 ccccggcctc cacctcctgc tcctgcccct cccgctcctg ctcctgctcc tgttccaccg
     8401 tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac accatctcta
     8461 tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt
     8521 cctcttcccc gtcctcgtcc atgtgccatg atggcggcct gcagctgtgt tcgaggccgc
     8581 gcgtgtcacc ttaatatgcg aagtggacct gggaccgcgc cgccccgact gcatctgcgt
     8641 gttcgagttc gccaatgaca agacgctggg cggggagatc ccccttatta accctaaacg
     8701 ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta attcaatagc
     8761 atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg
     8821 aacagcgatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     8881 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     8941 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     9001 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     9061 aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca
     9121 gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaagga gatctgtcga
     9181 catcgatggg cgcgggtgta cactccgccc atcccgcccc taactccgcc cagttccgcc
     9241 cattctccgc ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9301 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9361 aagctaattc ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9421 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9481 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9541 tgtagcaccg cctacatacc tcgctctgct gaagccagtt accagtggct gctgccagtg
     9601 gcgataagtc gtgtcttacc gggttggact caagagatag ttaccggata aggcgcagcg
     9661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta
     9961 gagtttaaac ttgacagatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10021 ggaaaagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10081 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgca gaagatcact
    10141 tgggtgcgcg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10201 ttcgccccga agaacgtttc ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10261 tattatcccg tattgatgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10321 atgacttggt tgaatactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10381 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10441 caactatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10501 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10561 ccacgatgcc tgtagcaatg gcaacaacgt tgcgaaaact attaactggc gaactactta
    10621 ctctagcttc ccggcaacaa ctaatagact ggatggaggc ggataaagtt gcaggaccac
    10681 ttctgcgctc ggcacttccg gctggctggt ttattgctga taaatcagga gccggtgagc
    10741 gtgggtcacg cggtatcatt gcagcactgg ggccggatgg taagccctcc cgtatcgtag
    10801 ttatctacac tacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10861 taggtgcctc actgattaag cattggtaag gataaatttc tggtaaggag gacacgtatg
    10921 gaagtgggca agttggggaa gccgtatccg ttgctgaatc tggcatatgt gggagtataa
    10981 gacgcgcagc gtcgcatcag gcattttttt ctgcgccaat gcaaaaaggc catccgtcag
    11041 gatggccttt cggcataact agtgaggctc cggtgcccgt cagt
//
