LOCUS       sequence-299341-       11087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Kalirin-7 with the c-front in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Miller MB, Yan Y, Wu Y, Hao B, Mains RE, Eipper BA
  TITLE     Alternate promoter usage generates two subpopulations of the 
            neuronal RhoGEF Kalirin-7.
  JOURNAL   J Neurochem. 2017 Mar;140(6):889-902. doi: 10.1111/jnc.13749. Epub 
            2016 Sep 6.
  PUBMED    27465683
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/jnc.13749";
            journalName: "J Neurochem"; date: "2017-03"; volume: "140"; issue: 
            "6"; pages: "889-902"
COMMENT     Sequence Label: pEAK-cKal7
FEATURES             Location/Qualifiers
     source          1..11087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          897..1100
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     1057..1077
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1117..1126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6458..6592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6495..6514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6549..6568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(6597..7196)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6680..6700)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7177..7196
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     rep_origin      9214..9349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9844..9863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(10032..10100,10101..10892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDHLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10250..10269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      complement(10890..11025)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga cgatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
      601 cccgtgcgtc ccagcgcaca tgcatgttcg gcgaggcggg gcctgcgagc gcggccaccg
      661 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
      721 ccgtgtatcg ccccgccctg ggcggcaagg ctgggagctc aaaatggagg acgcggcgct
      781 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
      841 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
      901 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
      961 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1021 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1081 gtttttttct tccatttcag gtgtcgtgaa aagcttgcca ccatgggaac ggaccgcttc
     1141 tgggaccagt ggtatctttg gtatctccgc ttgcttcggc ttctggatcg aggtatcctg
     1201 agatcttttc ggaatgatgg tttgaaagct tctgatgtcc ttcctatcct aaaggagaaa
     1261 gtggcctttg tgtctggggg tcgcgataag cgaggtggac ccatcctgac cttccctgcc
     1321 cgaagcaatc atgacagaat aagacaggaa gacctacgga aactcgtgac gtatttggcc
     1381 agcgtgccaa gcgaggacgt ttgcaaacgt ggcttcaccg tcatcatcga catgcggggc
     1441 tccaagtggg acctcatcaa gcccctcctc aagacgctgc aggaagcctt tcccgccgag
     1501 atccatgtgg ccctcatcat caagcctgac aacttctggc agaagcagaa gaccaacttt
     1561 ggcagctcca aattcatctt tgagacgagc atggtgtctg tggagggcct cacgaagctg
     1621 gtggacccct cccagctgac agaagagttt gacggttccc tggattacaa ccacgaggag
     1681 tggattgagt tgcgcctctc cctggaggag ttcttcaaca gtgcggtcca cctgctctca
     1741 cgcctcgagg acctgcagga gatgctggcc cggaaggagt tccctgtgga tgtggagggc
     1801 tctcggaggc ttatcgacga gcacacacaa cttaagaaaa aggtgctgaa ggcccccgtg
     1861 gaagagctgg accgggaggg gcagcggcta ctgcagtgta tccgctgcag tgatggcttc
     1921 tcagggcgca actgcatccc aggaagtgcc gacttccaga gccttgtacc caagatcacc
     1981 agcctcctgg acaagctgca ttccacccga caacacctgc accagatgtg gcacgtccga
     2041 aagctcaagc tggatcagtg cttccagctg cggctcttcg agcaggatgc cgagaagatg
     2101 ttcgactgga taagccacaa caaggagtta tttcttcaga gccacacaga gatcggagtc
     2161 agctaccagc atgcactaga cctccagaca cagcacaatc actttgccat gaactccatg
     2221 aatgcctatg tcaacatcaa ccgcatcatg tctgtcgcat cccgcctctc agaggctggc
     2281 cattatgcct cccagcaaat caagcagatc tccacgcagc tagaccagga gtggaagagc
     2341 tttgctgctg ccctggatga acgcagtaca atcctggcca tgtctgctgt gttccaccag
     2401 aaggcagagc agttcctgtc aggagtggat gcctggtgta agatgtgcag tgaaggcggc
     2461 cttccttctg agatgcagga cctggagctg gcaatccacc atcaccagtc cttgtatgag
     2521 caggtgaccc aggcctacac agaggtcagc caggatggga aagcactcct ggatgtcctg
     2581 cagcgtcccc tgagccctgg gaactccgag tccctcacag ccacagccaa ctactccaag
     2641 gcggtacacc aggtgctgga tgtggtacat gaggtgctgc accaccagcg gcggctcgag
     2701 agcatctggc agcaccgtaa agtgcggctt caccagcggc tgcagctgtg cgtcttccag
     2761 caggatgtgc agcaggtgct ggactggatt gaaaaccacg gtgaggcctt tctcagcaaa
     2821 cacactgggg tcggcaagtc cctgcatcgg gcacgggcat tgcagaagag gcatgatgac
     2881 tttgaagagg tggctcagaa tacatacacc aatgcggaca agctcctaga ggcagcagag
     2941 cagctggctc agactgggga atgtgacccg gaagagatct acaaggctgc ccgacacctg
     3001 gaggtgcgca tccaagactt cgtgcgcaga gtggagcagc ggaagcttct tctggacatg
     3061 tccgtctctt tccacacgca caccaaagag ctgtggacat ggatggaaga ccttcagaag
     3121 gaggtgctgg aggatgtctg tgcagactct gtggatgcgg tccaggaact gatcaagcag
     3181 ttccagcagc agcagaccgc taccctggac gccactctca acgtcatcaa ggaaggcgaa
     3241 gaccttatcc agcagctcag gtcagcgcct ccctccctgg gggagcccac cgaggccagg
     3301 gactcagctg tatccaacaa caagacgccc cacagtagtt ccatcagcca tatcgagtct
     3361 gtcctccagc agctggacga tgctcaggta cagatggagg agctgtttca cgagcggaag
     3421 atcaagctgg acatcttcct gcagctgcgc atcttcgaac agtacaccat tgaggtcacc
     3481 gcagagctgg atgcctggaa tgaggacttg ctccggcaga tgaatgactt caacacagag
     3541 gacctgaccc tggcagagca gcggctacag cgccacacag aacggaagct tgccatgaac
     3601 aacatgacat tcgaggtcat ccagcagggc caggacctgc accaatatat catggaagtc
     3661 caagcctcag gaatcgagtt aatctgtgag aaggacgttg acctggcagc tcaggtgcaa
     3721 gagctactgg aatttctcca tgagaagcag cacgaactgg agctcaatgc ggagcagacg
     3781 cacaagcggc tagagcagtg tctccagctg cggcacctcc aggctgaggt caaacaggtt
     3841 ctgggatgga tccgcaatgg agagtccatg ctcaacgcca gcctggtcaa tgccagctct
     3901 ttgtctgagg cagagcagct acagcgggag cacgaacagt tccagctagc catcgagaag
     3961 acgcaccaga gcgccctgca ggtacagcag aaggcagagg cactgctcca ggccggccac
     4021 tatgacgccg atgctatccg ggaatgtgcg gagaaggtgg ccctccactg gcaacagctc
     4081 atgctaaaga tggaggaccg gctgaaactc gtcaatgcct ctgtggcctt ttacaagacc
     4141 tctgagcagg tgtgcagcgt tctggagagc ttagagcagg agtaccggag agacgaagac
     4201 tggtgcggcg ggagagacaa gctggggccg gcggcagaga tggaccacgt cattccactc
     4261 ctcagcaagc atctggagca gaaggaggcc ttcctgaagg cctgcactct ggcacggcgg
     4321 aatgccgagg tgttcctcaa gtacatccac aggaacaatg tgagcatgcc cagtgttgcc
     4381 agccacacca ggggacccga acagcaagta aaagccatct tgagtgagct cttgcagaga
     4441 gagaatcgcg tcctgcactt ctggaccttg aagaagcggc ggttagacca gtgtcagcaa
     4501 tatgtggtgt ttgaacgcag tgctaaacag gcactagact ggatccaaga aacaggagaa
     4561 tattacctct caacccatac ctccactgga gagaccacag aggagactca ggagctgctg
     4621 aaagagtatg gagaattcag ggtgcctgcc aagcaaacaa aagagaaggt gaagcttctg
     4681 atccagctag cagacagctt tgtggaaaag ggccacatcc atgccacaga gatacggaaa
     4741 tgggtgacca cggtggacaa gcactaccgg gatttctccc tgaggatggg gaagtaccgg
     4801 tacaccctgg agaaagccct gggagtcaac acggaggata acaaggacct agagctggac
     4861 attatcccag caagcctttc agaccgggag gtcaagctcc gggatgctaa ccacgaggtc
     4921 aatgaagaga aacggaagtc agctcgaaag aaagaattca ttatggctga actgctccag
     4981 accgagaagg cttatgtaag agacctacac gagtgcttgg agacctacct gtgggaaatg
     5041 accagtggtg tggaagagat accacctggg atcctcaata aggagcacat catcttcggc
     5101 aacatccagg agatctatga cttccacaac aacatcttcc tcaaagagct ggagaagtac
     5161 gagcagctgc ctgaggatgt gggacactgc tttgttacct gggcagacaa atttcagatg
     5221 tatgtcacct actgtaaaaa caagcctgat tccaaccagc tgatcctgga gcacgcaggc
     5281 accttctttg atgagatcca gcagcggcac gggctggcca actccatctc ttcctactta
     5341 attaagcctg tccagagagt tacgaagtac cagctgctcc tgaaggaact tttaacttgc
     5401 tgtgaagaag ggaaagggga gctcaaggat ggcctggagg tgatgctcag tgtccccaag
     5461 aaagccaatg atgccatgca tgtcagcatg cttgaagggt tcgatgagaa cttggatgta
     5521 cagggggagt tgattctcca ggatgccttt caagtgtggg acccaaagtc gctgatccgg
     5581 aaggggcggg aacggcatct gttcctcttt gagatctcct tggtgtttag caaagagatc
     5641 aaagactctt caggacacac gaaatatgtt tacaagaaca agctactgac ctcagagctg
     5701 ggtgtgaccg agcacgtaga aggtgacccc tgcaaattcg ccttgtggtc tgggcgcacc
     5761 ccatcctcag acaataaaac agtgctcaaa gcttccaaca tcgaaaccaa gcaggaatgg
     5821 atcaagaaca ttcgggaagt gattcaggaa aggatcattc atcttaaagg agctttaaag
     5881 gagccaatcc agctccccaa aacaccagcc aaactgagga acaatagtaa gagggatggg
     5941 gtagaggatg gtgacagcca gggggacggg agcagccagc ccgataccat atccattgcc
     6001 tccaggacct ctcagaacac agtggagagt gacaaggatg gcaaccttgt tcctcggtgg
     6061 cacctgggac ctggagatcc tttctccact tacgtttagc gcgcagcctg ggacttgtcc
     6121 ctgcagctca ccgggttagc cgtctagagc ggccgcaggt aagccagccc aggcctcgcc
     6181 ctccagctca aggcgggaca ggtgccctag agtagcctgc atccagggac aggccccagc
     6241 cgggtgctga cacgtccacc tccatctctt cctcaggtct gcccgggtgg catccctgtg
     6301 acccctcccc agtgcctctc ctggtcgtgg aaggtgctac tccagtgccc accagccttg
     6361 tcctaataaa attaagttgc atcattttgt ttgactaggt gtccttgtat aatattatgg
     6421 ggtggaggcg ggtggtatgg agcaaggggc ccaagttaac ttgtttattg cagcttataa
     6481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgcttcag
     6601 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     6661 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
     6721 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     6781 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
     6841 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag cctggaaccg
     6901 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     6961 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     7021 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     7081 gggtcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     7141 gcccggggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggtg
     7201 gcctgcagag tcgctctgtg ttcgaggcca cacgcgtcac cttaatatgc gaagtggacc
     7261 tgggaccgcg ccgccccgac tgcatctgcg tgttttcgcc aatgacaaga cgctgggcgg
     7321 ggtttgtgtc atcatagaac taaagacatg caaatatatt tcttccgggg acaccgccag
     7381 caaacgcgag caacgggcca cggggatgaa gcagctgcgc cactccctga agatccatcg
     7441 tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc acctccttca
     7501 tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag
     7561 ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga
     7621 gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga
     7681 gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg
     7741 ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga
     7801 atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg
     7861 aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg
     7921 agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga
     7981 tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc cccgctgcgc
     8041 gggggcacgt caggctcacc atctgggccg ccttcttggt ggtattcaaa ataatcggct
     8101 tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg
     8161 gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc
     8221 cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct
     8281 ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct
     8341 cgaccccggc ctccacctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca
     8401 ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct
     8461 ctatgtcttg gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct
     8521 cgtcctcttc cccgtcctcg tccatgtgcc atgatggcgg cctgcagctg tgttcgaggc
     8581 cgcgcgtgtc accttaatat gcgaagtgga cctgggaccg cgccgccccg actgcatctg
     8641 cgtgttcgag ttcgccaatg acaagacgct gggcggggag atccccctta ttaaccctaa
     8701 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     8761 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     8821 aggaacagcg atctggatag catatgctat cctaatctat atctgggtag catatgctat
     8881 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     8941 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     9001 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     9061 cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag catatgatac
     9121 ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa ggagatctgt
     9181 cgacatcgat gggcgcgggt gtacactccg cccatcccgc ccctaactcc gcccagttcc
     9241 gcccattctc cgcctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9301 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9361 aaaaagctaa ttcggcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9421 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9481 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     9541 ctctgtagca ccgcctacat acctcgctct gctgaagcca gttaccagtg gctgctgcca
     9601 gtggcgataa gtcgtgtctt accgggttgg actcaagaga tagttaccgg ataaggcgca
     9661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcaag
     9961 ctagagttta aacttgacag atgagacaat aaccctgata aatgcttcaa taatattgaa
    10021 aaaggaaaag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10081 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gcagaagatc
    10141 acttgggtgc gcgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10201 gttttcgccc cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctatgtggcg
    10261 cggtattatc ccgtattgat gccgggcaag agcaactcgg tcgccgcata cactattctc
    10321 agaatgactt ggttgaatac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10381 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10441 tgacaactat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10501 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10561 acaccacgat gcctgtagca atggcaacaa cgttgcgaaa actattaact ggcgaactac
    10621 ttactctagc ttcccggcaa caactaatag actggatgga ggcggataaa gttgcaggac
    10681 cacttctgcg ctcggcactt ccggctggct ggtttattgc tgataaatca ggagccggtg
    10741 agcgtgggtc acgcggtatc attgcagcac tggggccgga tggtaagccc tcccgtatcg
    10801 tagttatcta cactacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10861 agataggtgc ctcactgatt aagcattggt aaggataaat ttctggtaag gaggacacgt
    10921 atggaagtgg gcaagttggg gaagccgtat ccgttgctga atctggcata tgtgggagta
    10981 taagacgcgc agcgtcgcat caggcatttt tttctgcgcc aatgcaaaaa ggccatccgt
    11041 caggatggcc tttcggcata actagtgagg ctccggtgcc cgtcagt
//
