LOCUS       sequence-29936-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     Rimm Plasmids
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BcatMutS33/S37.T41/S45
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             76..99
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             175..210
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctgcca ccatggacta caaggacgac gatgacaagg gacctaagct tggtaccgag
      121 ctcggatcca tggctactca agctgatttg atggagttgg acatggccat ggaaccagac
      181 agaaaagcgg ctgttagtca ctggcagcaa cagtcttacc tggacgctgg aatccatgct
      241 ggtgccactg ccacagctcc tgctctgagt ggtaaaggca atcctgagga agaggatgtg
      301 gatacctccc aagtcctgta tgagtgggaa cagggatttt ctcagtcctt cactcaagaa
      361 caagtagctg atattgatgg acagtatgca atgactcgag ctcagagggt acgagctgct
      421 atgttccctg agacattaga tgagggcatg cagatcccat ctacacagtt tgatgctgct
      481 catcccacta atgtccagcg tttggctgaa ccatcacaga tgctgaaaca tgcagttgta
      541 aacttgatta actatcaaga tgatgcagaa cttgccacac gtgcaatccc tgaactgaca
      601 aaactgctaa atgacgagga ccaggtggtg gttaataagg ctgcagttat ggtccatcag
      661 ctttctaaaa aggaagcttc cagacacgct atcatgcgtt ctcctcagat ggtgtctgct
      721 attgtacgta ccatgcaga
//
