LOCUS       sequence-299369-        2874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid with single copy of Mma PylT (CUA anticodon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pUC_h7SK Mma PylT (CUA)
FEATURES             Location/Qualifiers
     source          1..2874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..46)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      840..1428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1329..1348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1521..1538
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(2116..2163)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(2216..2235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2217..2235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2246..2263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2246..2262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2255..2277)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2578..2682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2683..2751,2752..2874,1..669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
       61 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      121 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      181 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      241 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      301 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
      361 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      421 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      481 gctggctggt ttattgctga taaatctgga gccggtgagc gtggttctcg cggtatcatt
      541 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      601 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      661 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      721 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      781 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      841 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      901 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      961 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1021 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1081 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1141 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1201 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1261 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1321 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1381 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1441 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcattaggc accccaggct
     1501 ttacccgaac gaccgagcgc agcgagtcag tgagcgagga agcggagagc gcccaatacg
     1561 caaggaaaca gctatgacca tgttaatgca gctggcacga caggtttccc gactggaaag
     1621 cgggcagtga gcggaaggcc catgaggcca gtcttgtgct ccattaatta atctcggcct
     1681 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     1741 ttaggggcgg gatagcactg actcctagga aaaaccgcac ttgtccggaa accccgggaa
     1801 tctaacccgg ctgaacggat ttagagtccg ttcgatctac atgatcaggt ttccgaggta
     1861 cccaggcggc gcacaagcta tataaacctg aaggaaatct caactttaca cttaggtcaa
     1921 gttacttatc gtactagagc ttcagcagga aatttaacta aaatctaatt taaccagcat
     1981 agcaaatatc atttattccc aaaatgctaa agtttgagat aaacggactt gatttccggc
     2041 tgttttgaca ctatccagaa tgccttgcag atgggtgggg catgctaaat actgcagaac
     2101 tagtgactga ctaaggaagt tcctatactt tctagagaat aggaacttcg gaataggaac
     2161 ttctagtggc gcgcctggac aagacacgtg gccttgacgg ccttccgcca attcgcccta
     2221 tagtgagtcg tattacgtcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2281 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2341 atagcgaaga ggcccgcacc gaaacgccct tcccaacagt tgcgcagcct gaatggcgaa
     2401 tgggagcgcc ctgtagcggc cactcaaccc tatctcggtc tattcttttg atttataagg
     2461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2521 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     2581 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2641 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2701 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2761 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2821 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg tttt
//
