LOCUS       sequence-299375-        2172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0366
FEATURES             Location/Qualifiers
     source          1..2172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..41
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(45..88)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      236..293
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(317..334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(488..1076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(568..587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1259..1353)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1374..2033)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1948..1967
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
       61 atccttagct ttcgctaagg atgatttctg gaattcggtc tcaggaggga ggcgtgtcgt
      121 tttagtgagg agcaagtgga tagcaagtgg atagcaagtg gatctatata aggttttgct
      181 attcattgaa agcagtagtg actgatttgt atataatgta ctcgagaccc tgcagtccgg
      241 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      301 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      361 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      421 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      481 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      541 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      601 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      661 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      721 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      781 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      841 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      901 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      961 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1021 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1081 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1141 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1201 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1261 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1321 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1381 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1441 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1501 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1561 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     1621 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1681 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1741 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1801 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     1861 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     1921 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     1981 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     2041 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     2101 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
     2161 agaaaccatt at
//
