LOCUS       sequence-299378-        2215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0368
FEATURES             Location/Qualifiers
     source          1..2215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..213
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(217..260)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      451..508
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(532..549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(703..1291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(783..802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1474..1568)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1589..2215,1..33))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2163..2182
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
       61 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
      121 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
      181 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
      241 ctttcgctaa ggatgatttc tggaattcgg tctcaggagg gaggcgtgtc gttttagtga
      301 ggatctctct gccgacagtg gtcccaaatc tctctgccga cagtggtccc aaatctctct
      361 gccgacagtg gtcccaaact atataaggtt ttgctattca ttgaaagcag tagtgactga
      421 tttgtatata atgtactcga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc
      481 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
      541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
      721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1141 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1261 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1321 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1381 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1441 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg
     1501 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     1561 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
     1621 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     1681 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     1741 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     1801 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     1861 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     1921 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     1981 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
     2041 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     2101 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     2161 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatc
//
