LOCUS       sequence-29938-e         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Jelezcova E, Trivedi RN, Wang XH, Tang JB, Brown AR, Goellner EM, 
            Schamus S, Fornsaglio JL, Sobol RW
  TITLE     Parp1 activation in mouse embryonic fibroblasts promotes Pol 
            beta-dependent cellular hypersensitivity to alkylation damage.
  JOURNAL   Mutat Res. 2010 Apr 1;686(1-2):57-67. doi: 
            10.1016/j.mrfmmm.2010.01.016. Epub 2010 Jan 22.
  PUBMED    20096707
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mutat 
            Res."; date: "2010-04-1"; volume: "686(1-2):57-67. doi"; pages: " 
            10.1016/j.mrfmmm.2010.01.016. Epub 2010 Jan 22"
COMMENT     Sequence Label: pIRES.Puro.EGFP
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(135..137,138..140,141..599)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(180..201)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(441..460)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttggtac cgagctcgga tcgatatcaa caagtttgta caaaaaagca ggctccgcgg
      121 ccgccccctt caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      181 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      241 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      301 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      361 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      421 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      481 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      541 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      601 ac
//
