LOCUS       sequence-299380-        2178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0380
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     143..162
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     390..408
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(412..455)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      609..666
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(690..707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(861..1449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(941..960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1632..1726)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1747..2178,1..228))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
       61 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
      121 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
      181 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
      241 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      301 gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa
      361 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat
      421 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcggtctca ggagggaggc
      481 gtgtcgtttt agtgagggtg ggagccacca gtgggagcca ccagtgggag ccaccactat
      541 ataaggtttt gctattcatt gaaagcagta gtgactgatt tgtatataat gtactcgaga
      601 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
      661 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      721 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      781 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      841 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      901 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      961 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1021 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1081 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1141 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1201 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1261 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1321 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1381 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1441 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1501 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1561 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1621 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1681 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1741 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1801 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1861 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1921 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1981 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2041 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     2101 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2161 cagctcaccg tctttcat
//
