LOCUS       sequence-299381-        2232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0381
FEATURES             Location/Qualifiers
     source          1..2232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      249..306
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(330..347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(501..1089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(581..600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1272..1366)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1387..2046)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1961..1980
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2208..2226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(join(2230..2232,1..41))
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg gtctcaggag
       61 ggaggcgtgt cgttttagtg aggtgaagat aagataataa tgttgaagat aagatgaaga
      121 taagataata atgttgaaga taagatgaag ataagataat aatgttgaag ataagactat
      181 ataaggtttt gctattcatt gaaagcagta gtgactgatt tgtatataat gtactcgaga
      241 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
      301 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      361 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      421 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      481 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      541 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      601 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      661 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      721 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      781 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      841 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      901 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      961 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1021 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1081 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1141 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1201 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1261 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1321 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1381 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1441 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1501 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1561 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1621 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1681 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1741 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1801 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1861 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1921 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1981 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2041 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2101 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     2161 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2221 cgaggcagaa tt
//
