LOCUS       sequence-299382-        2169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0372
FEATURES             Location/Qualifiers
     source          1..2169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     67..86
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     314..332
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(336..379)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      524..581
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(605..622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(776..1364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(856..875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1547..1641)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1662..2169,1..152))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
       61 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
      121 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
      181 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      241 cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac
      301 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
      361 tttcgctaag gatgatttct ggaattcggt ctcaggaggg aggcgtgtcg ttttagtgag
      421 gttttcaaca attttcaaca attttcaaca actatataag gttttgctat tcattgaaag
      481 cagtagtgac tgatttgtat ataatgtact cgagaccctg cagtccggca aaaaagggca
      541 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
      601 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      661 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      721 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      781 acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      841 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      901 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      961 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1021 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1081 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1141 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1201 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1261 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1321 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1381 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1441 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1501 tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa
     1561 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     1621 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     1681 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     1741 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     1801 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     1861 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     1921 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     1981 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     2041 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     2101 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     2161 ttgtgctta
//
