LOCUS       sequence-299383-        2184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0373
FEATURES             Location/Qualifiers
     source          1..2184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..60
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     288..306
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(310..353)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      513..570
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(594..611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(765..1353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(845..864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1536..1630)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1651..2184,1..126))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
       61 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      121 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      181 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
      241 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
      301 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
      361 cggtctcagg agggaggcgt gtcgttttag tgaggggaaa aagaagaggt ggaaaaagaa
      421 gaggtggaaa aagaagaggt ctatataagg ttttgctatt cattgaaagc agtagtgact
      481 gatttgtata taatgtactc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca
      541 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
      601 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      661 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      721 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
      781 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      841 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      901 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      961 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1021 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1081 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1141 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1201 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1261 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1321 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1381 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1441 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1501 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg
     1561 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     1621 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     1681 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     1741 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1801 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1861 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1921 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1981 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     2041 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     2101 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2161 tttctttacg gtctttaaaa aggc
//
