LOCUS       sequence-299385-        2178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0385
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     269..287
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(291..334)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      488..545
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(569..586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(740..1328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(820..839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1511..1605)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1626..2178,1..107))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
       61 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
      121 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
      181 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
      241 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
      301 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcggtctcag gagggaggcg
      361 tgtcgtttta gtgaggcagc cacttgtgtc agccacttgt gtcagccact tgtgtctata
      421 taaggttttg ctattcattg aaagcagtag tgactgattt gtatataatg tactcgagac
      481 cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
      541 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      601 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      661 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      721 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
      781 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      841 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      901 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      961 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1021 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1081 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1141 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1201 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1261 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1321 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1381 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1441 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     1501 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1561 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1621 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1681 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1741 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1801 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1861 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1921 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1981 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2041 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     2101 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2161 gtaatatcca gctgaacg
//
