LOCUS       sequence-299387-        2247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0387
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      253..310
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(334..351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(505..1093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(585..604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1276..1370)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1391..2050)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1965..1984
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2212..2230
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(join(2234..2247,1..30))
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagg gaggcgtgtc
       61 gttttagtga ggaagattga tgaaaagtca aaaacaaaaa tcaattataa gattgatgaa
      121 aagtcaaaaa caaaaatcaa ttataagatt gatgaaaagt caaaaacaaa aatcaattat
      181 ctatataagg ttttgctatt cattgaaagc agtagtgact gatttgtata taatgtactc
      241 gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
      301 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
      361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      481 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
      541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1261 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1321 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1381 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     1441 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     1501 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1561 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     1621 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1681 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1741 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1801 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     1861 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1921 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1981 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     2041 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     2101 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga
     2161 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     2221 atcacgaggc agaatttcag ataaaaa
//
