LOCUS       sequence-299388-        2172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0390
FEATURES             Location/Qualifiers
     source          1..2172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     115..133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(137..180)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      328..385
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(409..426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(580..1168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(660..679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1351..1445)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1466..2125)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2040..2059
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
       61 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      121 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
      181 tggaattcgg tctcaggagg gaggcgtgtc gttttagtga ggtcactatc agctcactat
      241 cagctcacta tcagcctata taaggttttg ctattcattg aaagcagtag tgactgattt
      301 gtatataatg tactcgagac cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg
      361 ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga
      421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc
      601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1021 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1321 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
     1381 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1441 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1501 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1561 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1621 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1681 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1741 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1801 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1861 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     1921 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1981 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     2041 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     2101 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     2161 caaaaaatac gc
//
