LOCUS       sequence-299390-        2181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0392
FEATURES             Location/Qualifiers
     source          1..2181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     209..228
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     456..474
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(478..521)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      678..735
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(759..776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(930..1518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1010..1029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1701..1795)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1816..2181,1..294))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
       61 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      121 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      181 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      241 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      301 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      361 attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct
      421 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt
      481 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg gtctcaggag
      541 ggaggcgtgt cgttttagtg aggcaaatat ttcttgtcaa atatttcttg tcaaatattt
      601 cttgtctata taaggttttg ctattcattg aaagcagtag tgactgattt gtatataatg
      661 tactcgagac cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct
      721 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
      781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      901 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
      961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1681 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     1741 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1801 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1861 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1921 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1981 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     2041 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     2101 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     2161 gagcgatgaa aacgtttcag t
//
