LOCUS       sequence-299396-        2217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0393
FEATURES             Location/Qualifiers
     source          1..2217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..181
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     409..427
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(431..474)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      667..724
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(748..765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(919..1507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(999..1018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1690..1784)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1805..2217,1..247))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
       61 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
      121 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
      181 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
      241 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
      301 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
      361 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
      421 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
      481 tcggtctcag gagggaggcg tgtcgtttta gtgaggttat gacccccgcc gatgacgcgg
      541 gattatgacc cccgccgatg acgcgggatt atgacccccg ccgatgacgc gggactatat
      601 aaggttttgc tattcattga aagcagtagt gactgatttg tatataatgt actcgagacc
      661 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
      721 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
      781 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      841 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      901 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
      961 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1021 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1081 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1141 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1201 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1261 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1321 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1381 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1441 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1501 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1561 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1621 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     1681 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1741 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1801 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1861 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1921 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1981 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     2041 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2101 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2161 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatca
//
