LOCUS       sequence-299400-        2176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0433
FEATURES             Location/Qualifiers
     source          1..2176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..121
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     349..367
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(371..414)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      566..623
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(647..664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(818..1406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(898..917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1589..1683)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1704..2176,1..187))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
       61 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
      121 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
      181 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
      241 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
      301 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
      361 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
      421 tcggtctcag gagggaggcg tgtcgtttta gtgaggtttt caacaaatcg aaaggacagt
      481 aggaaaaaga agaggtctat ataaggtttt gctattcatt gaaagcagta gtgactgatt
      541 tgtatatata ctcgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc
      601 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact
      661 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg
      841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1021 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1081 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1141 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1201 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1261 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1321 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1381 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1501 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1561 aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac
     1621 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     1681 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     1741 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     1801 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     1861 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     1921 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     1981 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2041 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2101 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2161 ttcatcaggc gggcaa
//
