LOCUS       sequence-299404-        2214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0388
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     365..383
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(387..430)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      620..677
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(701..718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(872..1460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(952..971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1643..1737)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1758..2214,1..203))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
       61 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
      121 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
      181 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
      241 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
      301 ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      361 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
      421 ggatgatttc tggaattcgg tctcaggagg gaggcgtgtc gttttagtga ggtccatcaa
      481 caaataatcc aagtaagtcc atcaacaaat aatccaagta agtccatcaa caaataatcc
      541 aagtaagcta tataaggttt tgctattcat tgaaagcagt agtgactgat ttgtatataa
      601 tgtactcgag accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt
      661 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc
      721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
      901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1621 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     1681 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     1741 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     1801 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     1861 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     1921 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     1981 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     2041 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     2101 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     2161 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agca
//
