LOCUS       sequence-299405-        2187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0440
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     144..163
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     391..409
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(413..456)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      619..676
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(700..717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(871..1459)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(951..970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1642..1736)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1757..2187,1..229))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
       61 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      121 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      181 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
      241 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      301 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa
      361 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
      421 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc aggagggagg
      481 cgtgtcgttt tagtgaggtc actatcagcc aaatatttct tgtttatgac ccccgccgat
      541 gacgcgggac tatataaggt tttgctattc attgaaagca gtagtgactg atttgtatat
      601 atactcgaga ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
      661 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
      721 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      781 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      841 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
      901 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      961 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1021 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1081 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1141 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1201 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1261 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1321 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1381 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1441 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1501 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1561 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1621 gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     1681 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     1741 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     1801 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     1861 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     1921 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1981 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     2041 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     2101 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     2161 cccatatcac cagctcaccg tctttca
//
