LOCUS       sequence-299406-        2209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0041
FEATURES             Location/Qualifiers
     source          1..2209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     339..358
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     586..604
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(608..651)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    654..675
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      836..893
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(917..934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1088..1676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1168..1187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1859..1953)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1974..2209,1..424))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
       61 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agtgatgaaa
      121 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
      181 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
      241 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
      301 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
      361 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
      421 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
      481 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
      541 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      601 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
      661 cggccgcttc tagagcgatg agtggtctcg ggagggaggc gtgtcgtttt agtgaggctg
      721 acgtaaggga tgacgcacac tatataaggt tttgctattc attgaaagca gtagtgactg
      781 atttgtatat aatgtacttg agaccactca tcgcgactag tagcggccgc tgcagtccgg
      841 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      901 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1081 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1501 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1861 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1921 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1981 ccgccctgcc actcgtcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     2041 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     2101 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2161 aaatcaaaac tggtgaaact cacccaggga ttggctgata cgaaaaaca
//
