LOCUS       sequence-299408-        2312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0310
FEATURES             Location/Qualifiers
     source          1..2312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     306..325
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     553..571
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(575..618)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    621..642
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      906..963
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(987..1004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1158..1746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1238..1257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1929..2023)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2044..2312,1..391))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
       61 cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
      121 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
      181 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
      241 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
      301 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
      361 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
      421 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      481 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
      541 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
      601 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt ggtctcggga
      661 gggaggcgtg tcgttttagt gaggtcattc aagatctctc tgccgacagt ggtcccaaag
      721 atggacccca agaggttcca accacgtcta caaagcaagt ggattgatgt gacatctcca
      781 ctgacgtaag ggatgacgca caatcccact atctatataa ggttttgcta ttcattgaaa
      841 gcagtagtga ctgatttgta tatatacttg agaccactca tcgcgactag tagcggccgc
      901 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
      961 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1021 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1081 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1141 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     1201 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1261 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1321 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1381 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1441 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1501 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1561 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1621 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1681 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1741 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1801 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1861 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     1921 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     1981 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     2041 aaattacgcc ccgccctgcc actcgtcgca gtactgttgt aattcattaa gcattctgcc
     2101 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     2161 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     2221 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgata cgaaaaacat
     2281 attctcaata aaccctttag ggaaataggc ca
//
