LOCUS       sequence-299409-        2337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0312
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     287..306
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     534..552
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(556..599)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    602..623
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      912..969
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(993..1010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1164..1752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1244..1263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1935..2029)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2050..2337,1..372))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
       61 tgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      121 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
      181 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      241 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      301 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      361 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      421 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
      481 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      541 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
      601 ggaattcgcg gccgcttcta gagcgatgag tggtctcggg agggaggcgt gtcgttttag
      661 tgaggtcatt caagatctct ctgccgacag tggtcccaaa gatggacccc aagaggttcc
      721 aaccacgtct acaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
      781 acaatcccac tattactgtt ggactctgaa gaagatacct atataaggtt ttgctattca
      841 ttgaaagcag tagtgactga tttgtatata tacttgagac cactcatcgc gactagtagc
      901 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      961 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1141 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     1201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1561 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1921 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1981 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     2041 gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat
     2101 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     2161 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     2221 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa
     2281 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgc
//
