LOCUS       sequence-299412-        2281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0225
FEATURES             Location/Qualifiers
     source          1..2281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     321..340
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     568..586
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(590..633)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      890..947
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(971..988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1142..1730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1222..1241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1913..2007)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2028..2281,1..406))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
       61 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
      121 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
      181 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
      241 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
      301 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
      361 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
      421 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
      481 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc
      541 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
      601 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cggtctcagg agggaggcgt
      661 gtcgttttag tgaggtatcg tcctttgaag ataagataat aatgttgaag ataagagtgg
      721 gagccaccac taaaacattg ctttgtcaaa agctaaaaaa gatgatgccc gacagccaga
      781 cgaaggaaat gacgtaagcc atgacgtcta atcccacaag ctatataagg ttttgctatt
      841 cattgaaagc agtagtgact gatttgtata tatactcgag accctgcagt ccggcaaaaa
      901 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
      961 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1141 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
     1921 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1981 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     2041 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2101 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2161 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2221 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     2281 t
//
