LOCUS       sequence-299414-        2331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0210
FEATURES             Location/Qualifiers
     source          1..2331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..173
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     401..419
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(423..466)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      773..830
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(854..871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1025..1613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1105..1124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1796..1890)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1911..2331,1..239))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
       61 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      121 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      181 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      241 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      301 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      361 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      421 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc
      481 aggagggagg cgtgtcgttt tagtgaggta tcgtcctttg aagataagat aataatgttg
      541 aagataagag tgggagccac cactaaaaca ttgctttgtc aaaagctaaa aaagatgatg
      601 cccgacagcc agacgaagga aatgacgtaa gccatgacgt ctaatcccac aagtttattg
      661 ggtacccgaa taactcagca tcttttgcta aattctatcg tggctatata aggttttgct
      721 attcattgaa agcagtagtg actgatttgt atatatactc gagaccctgc agtccggcaa
      781 aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg
      841 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1021 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct
     1801 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1861 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     1921 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1981 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     2041 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     2101 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     2161 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2221 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2281 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc a
//
