LOCUS       sequence-299415-        2356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0211
FEATURES             Location/Qualifiers
     source          1..2356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     184..203
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     431..449
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(453..496)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      828..885
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(909..926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1080..1668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1160..1179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1851..1945)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1966..2356,1..269))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
       61 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      121 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      181 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      241 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
      301 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
      361 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
      421 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
      481 cgctaaggat gatttctgga attcggtctc aggagggagg cgtgtcgttt tagtgaggta
      541 tcgtcctttg aagataagat aataatgttg aagataagag tgggagccac cactaaaaca
      601 ttgctttgtc aaaagctaaa aaagatgatg cccgacagcc agacgaagga aatgacgtaa
      661 gccatgacgt ctaatcccac aagaaagccc aggtcgtgtt gattcggatt ttgtaaaatg
      721 ggttctggcg tctttatgga ggttctccat ctgttttact atataaggtt ttgctattca
      781 ttgaaagcag tagtgactga tttgtatata tactcgagac cctgcagtcc ggcaaaaaag
      841 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
      901 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1081 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1801 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     1861 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1921 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1981 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2041 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2101 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2161 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     2221 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2281 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2341 gaaaacggtg taacaa
//
