LOCUS       sequence-299417-        2247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0212
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     153..172
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     400..418
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(422..465)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      688..745
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(769..786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(940..1528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1020..1039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1711..1805)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1826..2247,1..238))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
       61 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
      121 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
      181 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
      241 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
      301 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
      361 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
      421 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcggtctca
      481 ggagggaggc gtgtcgtttt agtgaggctg gagtttaatg agctaagcac atacgtcaga
      541 aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa atatactgtt
      601 ggactctgaa gaagatacct atataaggtt ttgctattca ttgaaagcag tagtgactga
      661 tttgtatata tactcgagac cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg
      721 ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga
      781 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      841 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc
      961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1381 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1501 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1621 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1681 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
     1741 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1801 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1861 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1921 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1981 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     2041 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     2101 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     2161 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     2221 gaacactatc ccatatcacc agctcac
//
